| 稻瘟病菌REMI突变体的筛选和鉴定 |
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| 引用本文： | 熊如意,刘娟,周益军,范永坚,郑小波.稻瘟病菌REMI突变体的筛选和鉴定[J].扬州大学学报(农业与生命科学版),2006,27(2):91-94. |
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| 作者姓名： | 熊如意 刘娟 周益军 范永坚 郑小波 |
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| 作者单位： | 1. 江苏省农业科学院,植物保护研究所,江苏,南京,210014
2. 南京农业大学,植物保护学院,江苏,南京,210095 |
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| 基金项目： | 国家自然科学基金;国家专项基金 |
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| 摘 要： | 利用构建的含潮霉素(HygB)抗性标记的质粒pUCATPH在稻瘟病菌菌株M 131中建立一个转化体系,通过限制酶介导整合(REM I)插入诱变技术,以HygB抗性作为突变体筛选标记,获得639个转化子。对其中200个转化子进行表型和致病性测定后,通过点杂交鉴定分别获得6个致病性突变菌株和部分表型突变菌株。对2个表型突变菌株和rep-PCR图谱差异性较大的2个致病性突变菌株进行RFLP分析,结果表明:突变体中均已插入质粒,但转化质粒在M 131中整合具有一定的随机性。
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| 关 键 词： | 稻瘟病菌 REMI 致病性突变 点杂交 rep-PCR RFLP |
| 文章编号： | 1671-4652(2006)02-0091-04 |
| 修稿时间： | 2005年2月28日 |
Screen and identification of mutants by REMI of Magnaporthe grisea |
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| XIONG Ru-yi,LIU Juan,ZHOU Yi-jun,FAN Yong-jian,ZHENG Xiao-bo.Screen and identification of mutants by REMI of Magnaporthe grisea[J].Journal of Yangzhou University:Agricultural and Life Science Edition,2006,27(2):91-94. |
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| Authors: | XIONG Ru-yi LIU Juan ZHOU Yi-jun FAN Yong-jian ZHENG Xiao-bo |
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| Abstract: | The plasmid pUCATPH was used to establish a transformation system in isolate M131 of Magnaporthe grisea.639 transformants were obtained by restriction enzyme mediated integration with Hygromycin B resistance as a tag.Two phenotype mutants and six pathogenicity mutants were screened out respectively,through the detection of the phenotype and pathogenicity and dot blot of 200 transformants.rep-PCR and RFLP analyses were done to study the mutation on molecular level and the integration sites of the plasmid DNA in the four mutants.Result showed that plasmid was inserted in all mutants and the conformity was random. |
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| Keywords: | Magnaporthe grisea REMI pathogenicity mutation dot blot rep-PCR RFLP |
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