| 降解纤维素嗜热真菌的筛选与分子鉴定 |
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| 引用本文： | 林白雪,刘斌,张宁宁,谢联辉. 降解纤维素嗜热真菌的筛选与分子鉴定[J]. 福建农林大学学报(自然科学版), 2011, 0(2): 182-186 |
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| 作者姓名： | 林白雪 刘斌 张宁宁 谢联辉 |
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| 作者单位： | 福建农林大学植物病毒研究所;福建农林大学食品科学学院; |
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| 基金项目： | 福建省自然科学重点基金项目(2007J0004) |
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| 摘 要： | 为获得有强降解纤维素能力的嗜热真菌,以羧甲基纤维素钠培养基为基础培养基,从温泉中初筛获得23株透明水解圈较大的降解纤维素的嗜热真菌.经过液体发酵复筛得到12株降解能力较强的优良菌株.通过18S rDNA序列分析,对之中的7株嗜热真菌进行分子鉴定,结果表明,7株嗜热真菌分别属于曲霉属、枝孢霉属和毛孢酵母属.
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| 关 键 词： | 纤维素酶 嗜热真菌 筛选 分子鉴定 |
Isolation and identification of thermophilic cellulolytic fungi |
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| LIN Bai-xue,LIU Bin,ZHANG Ning-ning,XIE Lian-hui. Isolation and identification of thermophilic cellulolytic fungi[J]. Journal of Fujian Agricultural and Forestry University, 2011, 0(2): 182-186 |
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| Authors: | LIN Bai-xue LIU Bin ZHANG Ning-ning XIE Lian-hui |
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| Affiliation: | LIN Bai-xue1,LIU Bin1,2,ZHANG Ning-ning1,XIE Lian-hui1 (1.Institute of Plant Virology,2.College of Food Science,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China) |
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| Abstract: | To obtain effective cellulose-decomposing fungi,sodium carboxymethyl cellulose was used as media to isolate and screen.Twenty-three thermophilic cellulolytic fungi strains were isolated from hot spring,indicated by large and distinct red circles around the colonies in Congo red agar medium.Twelve strains of fungi which had better capacity to decompose cellulose and hemicellulose were obtained through determinations of enzyme activity using liquid fermentation.Seven thermophilic fungi strains were identified... |
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| Keywords: | cellulase thermophilic fungi screening identification |
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