| 同源四倍体青花菜花粉活力及结籽性研究 |
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| 引用本文： | 张蜀宁,张杰,孙成振,王建军.同源四倍体青花菜花粉活力及结籽性研究[J].福建农业学报,2011,26(2):238-242. |
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| 作者姓名： | 张蜀宁 张杰 孙成振 王建军 |
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| 作者单位： | 南京农业大学作物遗传与种质创新国家重点实验室农业部南方蔬菜遗传改良重点开放实验室;云南农业大学园艺系; |
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| 基金项目： | 江苏省科技支撑计划项目(BE201130172) |
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| 摘 要： | 以二、四倍体青花菜为材料,采用TTC法、离体和原位萌发法研究其花粉活力,并用正交设计法研究其结籽性.结果表明:1) TTC染色试验中四倍体与二倍体花粉可染率分别为48.6%和60.8%,差异显著.2)离体萌发试验中4X花粉在4 h时萌发率最大,达66.45%,4 h后萌发率稳定;2X在5h时萌发率最大,达69.4%,5...
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| 关 键 词： | 青花菜 同源四倍体 花粉活力 结籽指数 |
Pollen Viability and Seed Formation of Autotetraploid Broccoli |
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| ZHANG Shu-ning,ZHANG Jie,SUN Cheng-zhen,WANG Jian-jun.Pollen Viability and Seed Formation of Autotetraploid Broccoli[J].Fujian Journal of Agricultural Sciences,2011,26(2):238-242. |
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| Authors: | ZHANG Shu-ning ZHANG Jie SUN Cheng-zhen WANG Jian-jun |
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| Institution: | ZHANG Shu-ning1,ZHANG Jie1,2,SUN Cheng-zhen1,WANG Jian-jun1(1.State Key Laboratory of Crop Genetics and Germplasm Enhancement,Nanjing Agricultural University,Nanjing,Jiangsu 210095,China,2.Department of Horticulture,Yunnan Agricultural University,Kunming,Yunnan 650201,China) |
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| Abstract: | Diploid and autotetraploid broccoli plants' pollen viabilities were studied by using TTC staining,in vitro as well as in situ germination,and their seed formation abilities by orthogonal experimentation.The results showed that: 1) the pollen TTC staining rates of the tetraploid and the diploid were 48.6% and 60.8%,respectively,which were significantly different;2) the in vitro pollen germination rate of the autotetraploid reached a maximum of 66.45% in 4h and leveled off afterward,while that of the diploid ... |
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| Keywords: | broccoli autotetraploid pollen viability seed formation index |
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