| 蜜蜂球囊菌分泌多种胞外酶侵染蜜蜂幼虫 |
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| 引用本文： | 郑志阳,李江红,梁勤,陈大福.蜜蜂球囊菌分泌多种胞外酶侵染蜜蜂幼虫[J].福建农林大学学报(自然科学版),2011(3):280-284. |
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| 作者姓名： | 郑志阳 李江红 梁勤 陈大福 |
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| 作者单位： | 福建农林大学蜂学学院; |
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| 基金项目： | 国家自然科学基金资助项目(30600454); 现代农业产业技术体系建设专项资金资助项目(CARS-45-KXJT) |
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| 摘 要： | 对健康和患病蜜蜂幼虫血淋巴进行SDS-PAGE电泳和蛋白酶、酯酶的活性染色,以研究蜜蜂球囊菌分泌的胞外酶种类及对蜜蜂侵染的作用.结果表明:健康蜜蜂幼虫血淋巴中的蛋白含量丰富,主要由4种高分子质量的蛋白组成,而患病幼虫血淋巴中的蛋白含量很少,主要蛋白组分被降解;在患病幼虫血淋巴中可以检测到健康幼虫所没有的多种蛋白酶和酯酶...
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| 关 键 词： | 蜜蜂球囊菌 侵染 胞外酶 血淋巴 活性染色 |
Ascosphaera apis secretes multiple extracellular enzymes to infect honeybee larvae |
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| ZHENG Zhi-yang,LI Jiang-hong,LIANG Qin,CHEN Da-fu.Ascosphaera apis secretes multiple extracellular enzymes to infect honeybee larvae[J].Journal of Fujian Agricultural and Forestry University,2011(3):280-284. |
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| Authors: | ZHENG Zhi-yang LI Jiang-hong LIANG Qin CHEN Da-fu |
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| Institution: | ZHENG Zhi-yang,LI Jiang-hong,LIANG Qin,CHEN Da-fu(College of Bee Science,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China) |
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| Abstract: | Bee chalk brood disease is one of the primary honeybee disease.It is caused by a entopathogenic fungi,Ascosphaera apis.Extracellular enzymes of entopathogenic fungi are often involved in host infecting process and therefore are vital pathogenic factors.Which extracellular enzymes were secreted by A.apis to infect honeybee is not clear so far.Here we collect the hemolymph from the healthy bee larvae and the A.apis infected larvae for SDS-PAGE,protease and esterase zymogram analysis.The result showed that the... |
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| Keywords: | Ascosphaera apis infection extracellular enzymes hemolymph zymogram |
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