| 高温对小菜蛾caspase-9酶活性和基因组DNA的影响 |
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| 引用本文： | 庄华梅,荆玉谱,张林杰,汪宽福,李晓辉,陈建丽,吴刚. 高温对小菜蛾caspase-9酶活性和基因组DNA的影响[J]. 福建农林大学学报(自然科学版), 2011, 0(3): 237-241 |
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| 作者姓名： | 庄华梅 荆玉谱 张林杰 汪宽福 李晓辉 陈建丽 吴刚 |
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| 作者单位： | 福建农林大学植物保护学院;福建农林大学生命科学学院; |
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| 基金项目： | 国家自然科学基金资助项目(30971924) |
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| 摘 要： | 研究了不同温度下不同时间处理对小菜蛾caspase-9蛋白酶活性和基因组DNA的影响.结果表明,40、42、45、47℃4个温度处理均能在短时间内使caspase-9蛋白酶活性显著增强;此外,33.5和40℃处理72 h可导致小菜蛾基因组DNA显著降解,电泳时呈现细胞凋亡的特征条带.高温能够诱导小菜蛾细胞凋亡.
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| 关 键 词： | 小菜蛾 细胞凋亡 caspase-9酶 |
Effects of high temperature on caspase-9 activity and DNA in genome of Plutella xylostella |
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| ZHUANG Hua-mei,JING Yu-pu,ZHANG Lin-jie,WANG Kuan-fu,LI Xiao-hui,CHEN Jian-li,WU Gang. Effects of high temperature on caspase-9 activity and DNA in genome of Plutella xylostella[J]. Journal of Fujian Agricultural and Forestry University, 2011, 0(3): 237-241 |
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| Authors: | ZHUANG Hua-mei JING Yu-pu ZHANG Lin-jie WANG Kuan-fu LI Xiao-hui CHEN Jian-li WU Gang |
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| Affiliation: | ZHUANG Hua-mei1,JING Yu-pu2,ZHANG Lin-jie2,WANG Kuan-fu2,LI Xiao-hui2,CHEN Jian-li2,WU Gang1(1.College of Plant Protection,2.College of Life Science,Fujian Agricultrue and Forestry University,Fuzhou,Fujian 350002,China) |
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| Abstract: | Effects of temperature and time on caspase-9 activity and DNA in genome of adult diamondback moth(DBM) was studied.The caspase-9 enzyme activity increased significantly when the adult DBM was pretreated with high temperature(40,42,45,47 ℃) in short time.Heat stress could cause DNA fragmentation,and the apoptotic DNA ladder fragments could be visualized by agarose gel.These results suggested that heat stress could induce apoptosis in DBM. |
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| Keywords: | Plutella xylostella apoptosis caspase-9 enzyme |
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