柞蚕酯酶同工酶的研究

应用聚丙烯酰胺凝胶电泳方法,调查了柞蚕酯酶同工酶在幼虫不同发育时期、不同组织器官及不同品种间的特异性变化:柞蚕酯酶同工酶活性和酶带数在幼虫不同发育时期存在一定的差异性,各龄盛食期酶带数目多且活性强,其表达与幼虫的生长发育有关;幼虫不同组织器官中酯酶同工酶的活性及种类差异很大,在脂肪体及中肠组织中活性较强,并且种类较多,酶活性的高低与组织的功能关系密切;酯酶同工酶在柞蚕品种间表现出很强的特异性,但在迁移率0.65处各品种均有1条共有谱带。UPGMA法聚类分析显示:33个柞蚕品种可分为4个大的类群,各品种与其母系亲本更多地表现出较近的亲缘关系,说明母系亲本在柞蚕杂交分离育种中起着决定性作用,所以杂交育种应优先注重对母系亲本性状的选择;部分品种在系统分离育种过程中来自亲本的一些性状逐渐弱化,而某一特定性状得到加强,与其亲本间表现出较远的亲缘关系。

Study on the Esterase Isozyme of Tussah Silkworm, Antheraea pernyi

Esterase isozyme of Tussah silkworm was investigated using PAGE method concerning different developing stages, organs and varieties. Activity and the kinds of esterase in the midgut varied among different developing stages. The activity and numbers of esterase loci were both higher in massive ingestion stage than that in other stages, suggested that the expression of esterase isozyme related to the development of Tussah silkworm. The kinds and activity of esterase varied greatly among different organs of Tussah silkworm larva. Esterase in fat body and midgut had higher activity and more kinds than that in other organs, indicated that the activity of esterase isozyme was closely related to the function of specific organ. The esterase isozymes were expressed differently among different varieties, but all the varieties shared a common esterase loci at Rf 0.65. The result of clustering analysis with UPGMA method showed that 33 Tussah varieties were classified into 4 groups, most varieties had closer relationship with their female parents, indicated that female parents play an important role in line separation breeding. The result suggested that more attention should be paid on the selection of female parents during the process of hybrid breeding. For some of the varieties bred by system separation method, part of their characters that come from their parent line may tapered gradually during long term intensive selection while some specific characters strengthened, resulted in the far blood relationship with their parents.