Affiliation: | a Dipartimento di Sanità e Benessere animale, Facoltà di Medicina Veterinaria, Str. Provinciale per Casamassima Km 3, I-70010 Valenzano (Bari), Italy b Dipartimento di Scienze Veterinarie e Agroalimentari, Facoltà di Medicina Veterinaria, Teramo, Italy |
Abstract: | The cytochrome oxidase I (COI) gene of the most wide-spread Italian species of Oestridae larvae causing myiasis (Gasterophilus spp., Hypoderma bovis, Hypoderma lineatum, Oestrus ovis and Przhevalskiana silenus) was amplified by polymerase chain reaction (PCR) using conserved primers. Restriction fragment length polymorphism (RFLP) of amplicons was also carried out and their restriction profiles compared. A clear genetic difference between the Oestridae larvae examined was demonstrated by using Taq I, Hinf I, Rsa I and Hpa II enzymes. No intra-specific variation in RFLPs was detected between the two species of Hypoderma. The results highlight the taxonomic and phylogenetic relationships among larvae belonging to the different subfamilies, and thus offer additional diagnostic and epidemiological instruments. |