| 苦荞胰蛋白酶抑制剂纯化方法的研究 |
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| 引用本文： | 杨致荣,杨斌,王转花. 苦荞胰蛋白酶抑制剂纯化方法的研究[J]. 山西农业大学学报(自然科学版), 2003, 23(1): 90-92 |
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| 作者姓名： | 杨致荣 杨斌 王转花 |
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| 作者单位： | 1. 山西农业大学,文理学院,山西,太谷,030801
2. 山西大学生命科学系,山西,太原,030006 |
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| 摘 要： | 以苦荞种子为材料,经热变性、硫酸铵盐析后制成苦荞胰蛋白酶抑制剂(TBTI)粗品,然后用离子交换层析及亲和层析的不同洗脱体系对其进行纯化。结果发现:亲和层析法操作简单、纯化效率高。十二烷基硫酸钠一聚丙烯酰胺凝胶电泳(SDS-PAGE)显示为一条带。分子量约为12 kDa～15 kDa。
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| 关 键 词： | 苦荞 胰蛋白酶抑制剂 分离 纯化 |
| 文章编号： | 1671-8151(2003)01-0090-03 |
| 修稿时间： | 2001-10-10 |
Studies on the Method of Purifying Typsin Inhibitor in Tartary Buckwheat by Chromatography |
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| YANG Zhi-rong et al.. Studies on the Method of Purifying Typsin Inhibitor in Tartary Buckwheat by Chromatography[J]. Journal of Shanxi Agricultural University(Nature Science Edition), 2003, 23(1): 90-92 |
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| Authors: | YANG Zhi-rong et al. |
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| Abstract: | A crude extract of typsin inhibitor from tatary buckwheat was obtained by heated treatment and precipitating with ammonium sulfate. Then the material was purified by ion-exchange and affinity chromatography using different e-luting buffer. The result showed that the purified TBTI by affinity chromatography was simple and highly efficient. The approximate molecular weight was 12 kDa-15 kDa by SDS-poly acrylamid gel-electrophoresis. |
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| Keywords: | Tatary buckwheat Typsin inhibition Isolation Purification |
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