| 橡胶HbJAZ1基因的原核表达与纯化分析 |
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| 引用本文： | 刘伟,翟金玲,许慧敏,黄惜. 橡胶HbJAZ1基因的原核表达与纯化分析[J]. 热带生物学报, 2011, 0(2): 117-122 |
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| 作者姓名： | 刘伟 翟金玲 许慧敏 黄惜 |
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| 作者单位： | 海南省热带生物资源可持续利用重点实验室／海南大学农学院生物科学技术研究所,海南海口570228 |
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| 基金项目： | 国家自然科学基金项目（31060107）; 教育部高等学校博士学科点专项科研基金博导类项目（20104601110003）; 海南大学211工程项目“热带作物遗传育种与生态保育”经费资助 |
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| 摘 要： | 为了鉴定巴西橡胶树中存在与JAZ蛋白特异性结合的蛋白,利用pGEX-6p-1载体构建橡胶JAZ（HbJAZ1）与GST（Glutathione S-transferase）融合表达载体,并成功转化大肠杆菌进行原核表达,同时利用谷胱甘肽-琼脂糖填料成功纯化gcHbJAZ1-GST融合表达蛋白。在纯化实验中,发现HbJAZ1融合表达蛋白在大肠杆菌原核表达中是难溶蛋白,大多存在于裂解菌液的沉淀物中。
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| 关 键 词： | 巴西橡胶树 JAZ 原核表达 蛋白纯化 |
Prokaryotic Expression and Purification of HbJAZ1 Protein of Hevea brasiliensis |
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| LIU Wei,ZHAI Jin-ling,XU Hui-min,HUANG Xi. Prokaryotic Expression and Purification of HbJAZ1 Protein of Hevea brasiliensis[J]. Journal of Tropical Biology, 2011, 0(2): 117-122 |
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| Authors: | LIU Wei ZHAI Jin-ling XU Hui-min HUANG Xi |
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| Affiliation: | (Key Laboratory of Tropic Biological Resources of Ministry of Education/Institute of Bio-Science and Technology, Hainan University,Haikou 570228,China) |
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| Abstract: | In order to identify the proteins in Hevea that interacted specifically with JAZ,the recombinant plasmid that expressed fusion protein of Hevea JAZ（HbJAZ1） and GST（Glutathione S-transferase） was constructed and transformed into Escherichia coli（E.coli） for prokaryotic expression and further purification.The results indicated that HbJAZ1-GST fusion protein was successfully expressed and purified,and the fusion proteins exist in pellet of bacterial lysis suspension. |
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| Keywords: | Hevea brasiliensis JAZ prokaryotic expression protein purification |
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