| 棉花蔗糖合成酶3基因第1个内含子在基因表达中的作用 |
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| 引用本文： | 阮孟斌,于晓玲,彭明.棉花蔗糖合成酶3基因第1个内含子在基因表达中的作用[J].热带生物学报,2011(4):291-296. |
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| 作者姓名： | 阮孟斌 于晓玲 彭明 |
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| 作者单位： | 中国热带农业科学院热带生物技术研究所,海南海口571101 |
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| 基金项目： | 国家转基因生物新品种培育重大专项资助项目（2009ZX08005-027B） |
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| 摘 要： | 为了研究棉花SUS3基因编码区的第1个内含子在基因表达调控中的作用,分离该内含子并将其插入到GUS基因编码区中构建载体Susfig::121,将载体Susfig::121通过农杆菌介导转入拟南芥.对转基因拟南芥进行GUS活性分析,发现该内含子对GUS基因在拟南芥中的表达具有负调控作用,并且特异抑制报告基因在花粉中表达....
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| 关 键 词： | 棉花 SUS3 内含子 基因表达调控 |
Function of the First Intron of Cotton Sucrose Synthase 3 for Expression Regulation |
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| RUAN Meng-bin,YU Xiao-ling,PENG ming.Function of the First Intron of Cotton Sucrose Synthase 3 for Expression Regulation[J].Journal of Tropical Biology,2011(4):291-296. |
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| Authors: | RUAN Meng-bin YU Xiao-ling PENG ming |
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| Institution: | (Chinese Academy of Tropical Agricultural Sciences, Institute of Tropical Bioscience and Biotechnology, Haikou 571101, China) |
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| Abstract: | Cotton sucrose synthase 3 （SUS3） plays an important role during the cotton fiber development. Compared to Arabidopsis SUS1/SUS4, the gene structure of cotton SUS3 has a high similarity with that of those two genes. To determine the function of the first intron of cotton SUS3, the intron was isolated and inserted into the GUS coding region to construct vector Susfig：121, and which was transformed into Arabidopsis thaliana mediated by agrobacterium. The results of GUS activity analysis of transgenic Arabidopsis plants indicated that the intron down-regulated GUS gene expression in non-pollen tissues and specifically repressed GUS expression in pollen, which suggested that the first intron played a role in gene expression. |
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| Keywords: | cotton SUS3 intron gene expression regulation |
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