| 水牛Cdc42cDNA的克隆与生物信息学分析 |
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| 引用本文： | 张晓茹,匡文华,成鹰,杜丽,雷明,焦寒伟,张冬琳,郝永昌,祁超,王凤阳.水牛Cdc42cDNA的克隆与生物信息学分析[J].热带生物学报,2011(4):349-354. |
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| 作者姓名： | 张晓茹 匡文华 成鹰 杜丽 雷明 焦寒伟 张冬琳 郝永昌 祁超 王凤阳 |
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| 作者单位： | [1]海南大学农学院／海南省热带动物繁育与疫病研究重点实验室（筹）／海口市动物基因工程重点实验室,海南海口570228 [2]华中师范大学生命科学学院,湖北武汉430079 |
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| 基金项目： | 国家转基因生物新品种培育重大专项（2009ZX08007-009B） |
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| 摘 要： | 通过RT-PCR扩增得到水牛细胞分裂周期蛋白42(Cdc42)cDNA序列,将扩增产物与pMD20-T载体连接,重组质粒经PCR、酶切鉴定后测序并进行生物信息学分析.结果表明:克隆得到的序列全长626 bp,含有1个大小为576 bp的开放阅读框,共编码191个氨基酸,相对分子质量为21.3×103,理论等电点为6.1...
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| 关 键 词： | Cdc42 水牛 基因克隆 生物信息学分析 |
Cloning and Sequence Analysis of Cdc42 cDNA from Buffalo |
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| ZHANG Xiao-ru,KUANG Wen-hua,CHENG Ying,DU Li,LEI Ming,JIAO Han-wei,ZHANG Dong-lin,HAO Yong-chang,QI Chao,WANG Feng-yang.Cloning and Sequence Analysis of Cdc42 cDNA from Buffalo[J].Journal of Tropical Biology,2011(4):349-354. |
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| Authors: | ZHANG Xiao-ru KUANG Wen-hua CHENG Ying DU Li LEI Ming JIAO Han-wei ZHANG Dong-lin HAO Yong-chang QI Chao WANG Feng-yang |
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| Institution: | 1.College of Agriculture,Hainan University,Hainan Key Lab of Tropical Animal Reproduction ＆ Breeding and Epidemic Disease Research (Construction Period),Animal Genetic Engineering Key Lab of Haikou,Haikou 570228,China; 2. College of Life Sciences,Huazhong Normal University,Wuhan 430079, China) |
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| Abstract: | The objective of the study was to clone full-length cDNA of Cdc42（Cell division cycle 42） gene of buffalo and analyze this gene.RT-PCR were performed to clone Cdc42 cDNA from buffalo, and the cloned Cdc42 cDNA sequence had been sent to GeneBank and got a accession number：JN380079.The bioinformatics analysis results showed that the Cdc42 cDNA sequence was 626 bp length,containing an open reading frame of 576 bp,encoding 191 amino acids.Relative molecular weight was 21.3 kD,pI was 6.16.Further analysis results indicated that the homology of Cdc42 gene of buffalo are 99.65%, 96.70%, 94.97% and 94.10%, compared to cattle, dog, mouse and human, respectively; the protein was non-transmembrane protein, which include a RHO domain. These results set a strong basis for further research of the function of Cdc42 of buffalo. |
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| Keywords: | Cdc42 Buffalo cloning bioinformatics analysis |
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