| 热带兰花2种病毒的ELISA检测 |
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| 引用本文： | 宋海超,张勇,邢波,谢玉成,刘福秀,史学群.热带兰花2种病毒的ELISA检测[J].热带生物学报,2011(2):148-152. |
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| 作者姓名： | 宋海超 张勇 邢波 谢玉成 刘福秀 史学群 |
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| 作者单位： | [1]海南大学环境与植物保护学院,海南海口570228 [2]海南出入境检验检疫局,海南海口570311 [3]三亚市柏盈热带兰花有限公司,海南三亚572029 [4]海口市美兰区科工信局,海南海口570203 |
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| 基金项目： | 海南省重大科技研发专项（080103）; 海南省自然科学基金（309138）; 国家科技富民强县项目之“兰花优良品种选育及无土栽种”标准化技术示范推广 |
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| 摘 要： | 采用三抗夹心酶联免疫吸附法（TAS-ELISA）和双抗夹心酶联免疫吸附法（DAS-ELISA）检测了海南主要兰花种植基地的疑似病毒感染的兰花叶片。结果表明,海南兰花病原病毒主要为建兰花叶病毒（Cym-bidium mosaic virus,CymMV）、齿兰环斑病毒（Odontoglossum ring-spot vi...
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| 关 键 词： | 兰花 建兰花叶病毒 齿兰环斑病毒 检测 |
ELISA Detection of Two Viruses in Tropical Orchid |
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| SONG Hai-chao,ZHANG Yong,XING Bo,XIE Yu-cheng,LIU Fu-xiu,SHI Xue-qun.ELISA Detection of Two Viruses in Tropical Orchid[J].Journal of Tropical Biology,2011(2):148-152. |
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| Authors: | SONG Hai-chao ZHANG Yong XING Bo XIE Yu-cheng LIU Fu-xiu SHI Xue-qun |
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| Institution: | 1.College of Environment and Plant Protection,Hainan University,Haikou 570228,China; 2.Hainan Entry-Exit Inspection and Quarantine Bureau,Haikou 570311,China; 3.Best Goldlines Tropical Orchid Industrial Co.,Ltd,Sanya 572029,China; 4.Bureau of Scientific and Technical Information,Meilan District Government,Haikou 570203,China) |
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| Abstract: | The TAS-ELISA and DAS-ELISA were established to detect the orchids suspected to be infected by virus mainly from main orchid planting in Hainan Province.The results suggested that the pathogen were Cymbidium mosaic virus（CymMV）,Odontolossum ring spot virus（ORSV） and their mixed infection;there were more orchids which were infected with CymMV than with ORSV.The method possesses high sensitivity,accuracy and specificity. |
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| Keywords: | orchid Cymbidium mosaic virus Odontoglossum ring-spot virus detection |
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