| 广藿香组织培养快繁技术的研究 |
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| 引用本文： | 曹嵩晓,李碧英,李娟玲,刘国民,韦江云. 广藿香组织培养快繁技术的研究[J]. 热带生物学报, 2011, 0(2): 143-147 |
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| 作者姓名： | 曹嵩晓 李碧英 李娟玲 刘国民 韦江云 |
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| 作者单位： | [1]海南大学苦丁茶研究所,海南海口570228 [2]海南大学应用科技学院（城西校区）,海南海口571101 |
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| 摘 要： | 以广藿香幼嫩叶片为外植体,通过愈伤组织途经诱导出大量丛生芽,然后将丛生芽分别转接到含有不同质量浓度6-BA的增殖培养基和含有不同质量浓度IBA的生根培养基上,筛选出最适增殖培养基：MS＋6-BA 0.1～0.2 mg.L-1,最适生根培养基：1/2 MS＋IBA 0.25～1.0 mg.L-1。广藿香组培苗株高达5～6 cm时进行炼苗移栽,移栽成活率高达95%。
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| 关 键 词： | 广藿香 丛生芽 组织培养 快繁技术 移栽 |
Rapid Propagation of Pogostemon cablin via Tissue Culture |
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| CAO Song-xiao,LI Bi-ying,LI Juan-ling,LIU Guo-min,WEI Jiang-yun. Rapid Propagation of Pogostemon cablin via Tissue Culture[J]. Journal of Tropical Biology, 2011, 0(2): 143-147 |
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| Authors: | CAO Song-xiao LI Bi-ying LI Juan-ling LIU Guo-min WEI Jiang-yun |
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| Affiliation: | 1.The Kudingcha Research Institute,Hainan University,Haikou 570228,China; 2.College of Application Science and Technology,Hainan University,Haikou 571101,China) |
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| Abstract: | The young leaves of Pogostemon cablin were used as explants,and a large number of shoots were obtained through inducing callus.The shoots were transferred onto the multiplication media with different concentration of 6-BA,and onto the root-promoting media with different concentration of IBA.The optimum multiplication culture medium was obtained（MS ＋0.1 mg·L-1—0.2 mg·L-1 6-BA）,and the optimum root-promoting medium was obtained（1/2 MS ＋0.25 mg·L-1—1.0 mg·L-1 IBA）.When the height of the plantlets of Pogostemon cablin amounted to 5—6 cm,the survival percentage of plantlets Pogostemon cablin in the transplantation was 95%. |
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| Keywords: | Pogostemon cablin clustered shoots rapid propagation via tissue culture transplantation |
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