Ethanolic fermentation of phosphoric acid hydrolysates from olive tree pruning |
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| Institution: | 1. Department of Chemical, Environmental and Materials Engineering, University of Jaén, 23071 Jaén, Spain;2. Department of Chemical Engineering, University of Granada, 18071 Granada, Spain;1. Department of Chemical, Environmental and Materials Engineering, University of Jaén, Campus ‘Las Lagunillas’, 23071 Jaén, Spain;2. Department of Chemical Engineering, University of Málaga, Campus ‘Teatinos’, 29071 Málaga, Spain;3. Renewable Resources Laboratory, Biotechnology Center, University of Concepción, Casilla 160-C, Concepción, Chile;1. Chemical Engineering Department, University of Vigo (Campus Ourense), Faculty of Science, Polytechnical Building, As Lagoas, 32004 Ourense, Spain;2. CITI (Centro de Investigación, Transferencia e Innovación), Universtity of Vigo, Tecnopole, San Cibrao das Viñas, 32900 Ourense, Spain;1. Research Center in Applied Chemistry (CEPESQ), Department of Chemistry, Federal University of Paraná (UFPR), P.O. Box 19032, Curitiba, PR 81531-980, Brazil;2. Department of Chemical Engineering, UFPR, Curitiba, PR 82530-990, Brazil;3. INCT in Energy and Environment (INCT E&A), UFPR, Curitiba, PR 81531-980, Brazil;1. Program in Environmental Science, Faculty of Science and Technology, Suratthani Rajabhat University, Surat Thani 84100, Thailand;2. Department of Industrial Biotechnology, Faculty of Agro-Industry, Prince of Songkla University, Songkhla 90112, Thailand;3. Palm Oil Products and Technology Research Center (POPTEC), Faculty of Agro-Industry, Prince of Songkla University, Songkhla 90112, Thailand;4. Department of Biology, Faculty of Science, Thaksin University, Phatthalung 93110, Thailand;5. Biological Systems Engineering, University of Wisconsin-Madison, 460 Henry Mall, Madison, WI 53706, United States;1. Institute of Ecological and Environmental Sciences, Sichuan Agricultural University-Chengdu Campus, Chengdu, Sichuan 611130, PR China;2. Engineering & Technology Center for Rural Environment Protection of Sichuan Province, Sichuan Agricultural University-Chengdu Campus, Chengdu, Sichuan 611130, PR China;3. Forest Products Biotechnology, Department of Wood Science, The University of British Columbia, 2424 Main Mall, Vancouver, BC, Canada;4. Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, Jiangsu 214122, PR China;5. Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, Jiangsu 214122, PR China |
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| Abstract: | The objective of this work was to study the feasibility of using phosphoric acid to hydrolyze the hemicellulosic fraction of olive tree pruning, as a step in the bioconversion process to produce ethanol. Milled olive tree pruning was submitted to hydrolysis at 90 °C by phosphoric acid in a concentration range 0.3–8N for 240 min. The hydrolysates were then fermented by Pachysolen tannophilus. The hydrolysis stage was evaluated by the evolution of glucose and reducing sugars generated and by the conversion of hemicellulose fraction. The main parameters determined in the fermentation were: maximum specific growth rate, specific substrate consumption rate, specific ethanol production rate and ethanol yield. The maximum ethanol yield (0.38 kg/kg, equivalent to 74.5% of the theoretical yield) was obtained when hydrolysing with 0.5N phosphoric acid. Hemicellulose conversion is however incomplete at these operational conditions. Higher acid concentrations lead to higher hydrolysis of hemicellulose, but the ethanol yields resulting from the fermentation are lower. |
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