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桉树扦插苗焦枯病病原鉴定
引用本文:陈全助,郭文硕,冯丽贞,缪三华,林家杉. 桉树扦插苗焦枯病病原鉴定[J]. 福建农林大学学报(自然科学版), 2013, 42(3)
作者姓名:陈全助  郭文硕  冯丽贞  缪三华  林家杉
作者单位:1. 福建农林大学林学院,福建福州,350002
2. 福建农林大学林学院,福建福州350002;福建农林大学森林保护研究所,福建福州350002
3. 永安市林业局,福建永安,366000
基金项目:福建省财政厅社会公益研究项目,福建省林业厅科技项目,福建省林业厅项目,福建省财政厅项目
摘    要:为了解丽赤壳属种的分布动态,科学防控焦枯病菌危害,经柯赫法则进行致病性验证,通过β-微管蛋白、组蛋白H3和翻译延伸因子-1α3个基因序列比对以及形态特征观察,对福建省苗圃地采集的尾细桉M1和巨桉5号扦插苗焦枯病菌进行鉴定.结果表明,供试菌株与已知种Calonectria kyotensis Terashita(无性型:Cylindrocladium floridanum Sobers&Seymour)的形态基本一致,且亲缘关系相近,将其鉴定为Ca.kyotensis.该菌能够侵染桉树引起焦枯病在国内为首次报道.

关 键 词:桉树  焦枯病  形态学特征  系统发育

Identification of Calonectria associated with Eucalyptus spp.cutting seedling leaf blight
CHEN Quan-zhu , GUO Wen-shuo , FENG Li-zhen , MIAO San-hua , LIN Jia-shan. Identification of Calonectria associated with Eucalyptus spp.cutting seedling leaf blight[J]. Journal of Fujian Agricultural and Forestry University, 2013, 42(3)
Authors:CHEN Quan-zhu    GUO Wen-shuo    FENG Li-zhen    MIAO San-hua    LIN Jia-shan
Abstract:Species of Calonectria are well-known causal agents of Cylindrocladium leaf blight.For the purpose of knowing the distribution dynamic of Calonectria,and guiding the prevention and control of Cylindrocladium leaf blight scientifically,isolates collected from Eucalyptus grandis×Eucalyptus tereticornis M1 and Eucalyptus grandis 5 cutting production were identified using morphological characteristics and DNA sequence comparisons for the β-tubulin,histone H3 and translation elongation factor-1α genes.The result indicated that four isolates were proved to the pathogens of Cylindrocladium leaf blight by Koch′s postulates,and were identified as Ca.kyotensis Terashita(anamorph: Cy.floridanum Sobers & Seymour).Ca.kyotensis can infect Eucalyptus spp.and cause leaf blight was first reported in China.
Keywords:Eucalyptus spp.  Cylindrocladium leaf blight  morphological characteristics  phylogram
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