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银杏外种皮对小菜蛾生长及体内保护酶活性的影响
引用本文:唐进根,赵东亚,陈利红,曹福亮. 银杏外种皮对小菜蛾生长及体内保护酶活性的影响[J]. 福建农林大学学报(自然科学版), 2013, 42(3)
作者姓名:唐进根  赵东亚  陈利红  曹福亮
作者单位:南京林业大学森林资源与环境学院,江苏南京,210037
基金项目:国家林业公益性行业科研项目
摘    要:用12.5、25、50、100、200 mg.mL-1银杏外种皮提取物浸泡过的无农药残留的小青菜饲喂小菜蛾3龄幼虫,研究了银杏外种皮提取物对小菜蛾生长及其体内3种保护酶SOD、CAT、POD活性的影响.结果表明:银杏外种皮提取物对小菜蛾幼虫具有一定的拒食活性和毒杀作用,能够降低幼虫化蛹率,影响蛹的质量.此外,银杏外种皮提取物能够扰乱小菜蛾幼虫体内保护酶的动态平衡,主要表现为SOD活性被激活,4、36、48 h时处理组SOD活力均显著高于对照组;CAT活性被抑制,4、24 h时处理组CAT活力均显著低于对照组;4-36 h POD活性被抑制,处理组POD活力均显著低于对照组,而48 h时POD活性又被激活,处理组活力显著高于对照组.

关 键 词:银杏外种皮  小菜蛾  生物活性  过氧化物酶  过氧化氢酶  超氧化物歧化酶

Effects of Ginkgo biloba episperm on the growth and enzymatic activity of Plutella xylostella
TANG Jin-gen , ZHAO Dong-ya , CHEN Li-hong , CAO Fu-liang. Effects of Ginkgo biloba episperm on the growth and enzymatic activity of Plutella xylostella[J]. Journal of Fujian Agricultural and Forestry University, 2013, 42(3)
Authors:TANG Jin-gen    ZHAO Dong-ya    CHEN Li-hong    CAO Fu-liang
Abstract:The 3rd instar larvae of Plutella xylostella were fed with green Chinese cabbage without pesticide residues soaked in 12.5 mg·mL-1,25 mg·mL-1,50 mg·mL-1,100 mg·mL-1 and 200 mg·mL-1 extracts from Ginkgo biloba episperm,respectively,and effects of extracts on the growth and activities of three protective enzymes such as SOD,CAT and POD of P.xylostella larvae were studied in the laboratory.The results showed that the extracts had strong toxicity and antifeedant activity to the larvae and could reduce the pupation rate and the quality of pupa.In addition,the extracts could disturb the dynamic balance of protective enzymes.The SOD activity was activated significantly at 4 h,36 h and 48 h,while the CAT activity was restrained significantly at 4 h and 24 h.The POD activity was restrained significantly at 4 h,12 h,24 h and 36 h,then was activated significantly at 48 h.
Keywords:Ginkgo biloba episperm  Plutella xylostella  biological activity  POD  CAT  SOD
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