| 生乳与UHT灭菌乳中糠氨酸含量及其测定方法研究 |
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| 引用本文： | 王加启,卜登攀,于建国,刘仕军,魏宏阳.生乳与UHT灭菌乳中糠氨酸含量及其测定方法研究[J].中国奶牛,2005(6):13-15. |
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| 作者姓名： | 王加启 卜登攀 于建国 刘仕军 魏宏阳 |
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| 作者单位： | 中国农业科学院畜牧研究所,北京,100094 |
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| 摘 要： | 本文研究了生乳与UHT灭菌乳中糠氨酸含量及其测定方法,并提出糠氨酸作为UHT灭菌乳中复原乳成分的标示物质,以鉴定UHT灭菌乳中是否掺入复原乳。结果表明,生乳中糠氨酸含量低于7mg/hg(按蛋白质计);乳粉中糠氨酸含量大于135mg/hg,不含复原乳的UHT灭菌乳中糠氨酸含量应小于140mg/hg(按蛋白质计)。使用高效液相色谱(HPLC)紫外检测糠氨酸含量,平均偏差<5%(n=5);回收率为98.2%。
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| 关 键 词： | 生乳 UHT灭菌乳 HPLC 糠氨酸(furosine) |
| 文章编号： | 1004-4264（2005）06-0013-03 |
| 收稿时间： | 2004-07-06 |
| 修稿时间： | 2004年7月6日 |
The Study on Content and Analytical Methods of Furosine in Raw Milk and UHT Milk |
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| Wang Jiaqi,Bu Dengpan,Yu Jianguo,Liu Shijun,Wei Hongyang.The Study on Content and Analytical Methods of Furosine in Raw Milk and UHT Milk[J].China Dairy Cattle,2005(6):13-15. |
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| Authors: | Wang Jiaqi Bu Dengpan Yu Jianguo Liu Shijun Wei Hongyang |
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| Institution: | Institute of Animal Science and Technology, Chinese Academy of Agricultural Science, Beijing 100094, China |
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| Abstract: | The aim of this study was to establish a analytical method for furosine that determine whether reconstituted milk was interpolative in UHT milk, and measure furosine content in raw milk and UHT milk. The result proved that furosine may be as a identify marker in milk. In addition, the result showed that furosine content is below 7mg/hg protein in the raw milk, and is above 135mg/hg protein in milk powder. Furosine is not beyond 140mg/hg protein in UHT(ultra high-temperature sterilized) milk that has not powdered milk. At the same time, it is showed that mean deviation of analytical furosine content is less than 5% by HPLC(High Pressure Liquid Chromatography), and recovery ratio rate of standard furosine is 98.2 %. |
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| Keywords: | Raw milk UHT milk HPLC Furosine |
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