离体玉米茎尖直接产生雌、雄花序过程中内源激素的研究

Using ELISA(Enzyme Linked Immunosorbent Assay) methods,three-type phytohormone in cultured shoot apices of maize were determined.It showed that the contents of three kinds of endogenous cytokinins changed unbalanced,which including ZRs,DHZRs and iPAs,cytokinins kept in high contents totally compared to the contents of IAA and GA_1/3.The concentration of IAA decreased with the in-vitro culture time was postponed,specially at the time of the 35-40 days when the ears or tassels emerged from the in vitro cultured shoot apices.The contents of GA_1/3 increased steadily in all 40 days of the in-vitro culture progress,but the concentration of ABA kept in a very low level in all the in-vitro culture progress.These results exhibited that both GA_1/3 and three kinds of cytokinins played an important and positive role when the maize shoot apices changed from the vegetative developmental stage to the reproductive one.

Dynamic Changes of Endogenous Phytohormones in Ears and Tassels Which Emerged from Cultured Shoot Apices of Maize