| 高活性纤维素酶的分离纯化 |
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| 引用本文： | 李志平 杨盛 侯红萍. 高活性纤维素酶的分离纯化[J]. 中国农学通报, 2012, 28(18): 205-208. DOI: 10.11924/j.issn.1000-6850.2012-0555 |
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| 作者姓名： | 李志平 杨盛 侯红萍 |
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| 作者单位： | 山西省农科院果树研究所,山西太谷,030815 |
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| 基金项目： | 山西省科技厅攻关项目（041030） |
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| 摘 要： | 为了纯化纤维素酶粗酶液，通过采用硫酸铵分级沉淀、Sephadex G-100凝胶柱层析、DEAF FF弱阴离子交换柱层析的方法，研究纤维素酶粗酶液分离纯化的方法。结果表明：采用硫酸铵分级沉淀、Sephadex G-100凝胶柱层析、DEAF FF弱阴离子交换柱层析的方法对内切葡聚糖苷酶组分的分离纯化有效。通过试验研究得到一种内切葡聚糖苷酶组分，其比活力提高了5.89倍，回收率为22%。
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| 关 键 词： | 污染评价 污染评价 |
| 收稿时间： | 2012-02-23 |
| 修稿时间： | 2012-04-09 |
Purification of Highly Active Cellulase |
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| Li Zhiping , Yang Sheng , Hou Hongping. Purification of Highly Active Cellulase[J]. Chinese Agricultural Science Bulletin, 2012, 28(18): 205-208. DOI: 10.11924/j.issn.1000-6850.2012-0555 |
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| Authors: | Li Zhiping Yang Sheng Hou Hongping |
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| Affiliation: | (Pomology Institute,Shanxi Academy of Agricultural Sciences,Taigu Shanxi 030815) |
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| Abstract: | To study the separation and purification of cellulase from crude enzyme,the method of ammonium sulfate fractionation,Sephadex G-100 gel filtration and DEAF FF weak anion exchange column chromatography method were used.The results showed that：the usage of ammonium sulfate fractionation,Sephadex G-100 gel column chromatography,DEAF FF weak anion exchange column chromatography was a efficient method to purify CMCase.The specific activity of CMCase increased 5.89 times purifed in this way,while the recovery rate was 22%. |
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| Keywords: | cellulase； mixed strains； purification |
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