| 转基因克隆牛外源基因整合位点的研究 |
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| 引用本文： | 谭贝贝,苏红,胡嘉祺,吴茜红,徐大庆,巩校东,李世杰.转基因克隆牛外源基因整合位点的研究[J].中国农学通报,2012,28(17):28-32. |
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| 作者姓名： | 谭贝贝 苏红 胡嘉祺 吴茜红 徐大庆 巩校东 李世杰 |
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| 作者单位： | 1. 河北农业大学生命科学学院,河北保定,071001
2. 中国地质大学长城学院,河北保定,071000 |
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| 基金项目： | 转基因生物新品种培育重大专项“高通量基因克隆技术体系的研究”（2011ZX08009-002）;国家重大科技专项（2008zx08006-004）;国家自然科学基金“体细胞克隆牛Dlkl-Gtl2印记区域表观遗传重编程的研究”（30972098）. |
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| 摘 要： | 整合位点的侧翼序列是外源基因表型研究和功能探讨的前提条件，对目的基因的正常转录和表达有着重要影响。本试验利用热不对称交措式PCR（Thermal Asymmetric Interlaced PCR,TAIL-PCR）的方法克隆了目的基因。结果表明：外源基因整合到牛的12号染色体基因组克隆(NW_003104315.1)中。分析了整合位点的纯合性发现转基因牛为整合位点杂合子。TAIL-PCR法能够有效、快速的鉴定外源基因在基因组中的整合位置，具有良好的应用前景。
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| 关 键 词： | 化学品质 化学品质 |
| 收稿时间： | 2012/2/28 0:00:00 |
| 修稿时间： | 2012/4/17 0:00:00 |
TraTransgene Integration Site Analysis in Transgenic Bovine |
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| Tan Beibei
,
Su Hong
,
Hu Jiaqi
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Wu Xihong
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Xu Daqing
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Gong Jiaodong
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Li Shijie.TraTransgene Integration Site Analysis in Transgenic Bovine[J].Chinese Agricultural Science Bulletin,2012,28(17):28-32. |
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| Authors: | Tan Beibei Su Hong Hu Jiaqi Wu Xihong Xu Daqing Gong Jiaodong Li Shijie |
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| Institution: | (College of Life Science, Hebei Agriculture University of China, Baoding Hebei 071001; 2College of Great Wall, China University of Geosciences, Baoding Hebei 071000) |
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| Abstract: | It was important to detect transgene integration site after establishment of transgenic animal line, and it was necessary to determine transgene expression and function. The integration site was successfully amplified using Thermal Asymmetric Interlaced PCR （TAIL-PCR）. The results showed that the target sequence was integrated into chromosome 12 （NW_003104315.1）. The homozygosis analysis of integration site showed the transgenic bovine was heterozygosity. The transplanted gene could be easily and quickly located in the genomic sequence and TAIL-PCR has advanced future application. |
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| Keywords: | TAIL-PCR transgenic bovine integration site zygosity |
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