云南地方老品种水稻中恢复基因位点遗传差异分析

目前生产上使用的水稻恢复系之间的遗传基础过于狭窄，应积极开发水稻新恢复系的选育工作。云南地理气候条件特殊，地方老品种资源丰富，是筛选新恢复系的理想材料。以61份云南地方老品种水稻资源为父本，通过测交筛选到6份可以恢复野败型不育系的品种。再利用5对特异引物，对这6份恢复材料和7份恢复系的恢复基因位点遗传差异进行分析。结果表明：5对特异引物对6份恢复材料的扩增结果各不一样，而与对照恢复系仅有2.8 kb的一条扩增带型一致。说明云南地方老品种水稻中蕴藏的恢复基因与目前生产上应用的恢复基因的亲缘关系较远。从云南地方老品种水稻中发掘利用新型恢复基因和资源，是突破水稻恢复基因遗传基础狭窄、筛选新质源恢复系的一种有效策略。

Genetic Analysis of Rice Restorer Gene Locus in Yunnan Landrace

In hybrid rice breeding,new restore lines breed process should be promoted because of the restriction of the narrow genetic relationship.Yunnan Province,processing unique climate conditions,provided abundant rice landraces which was the ideal resources for new cytoplasm type restore lines selection.In our research,61 Yunnan landraces were used for test-crossing,and 6 lines were selected through the fertility restorer ability.Moreover,5 specific primers which located inside restore gene locus were used for analyzing the genetic diversity of restorer gene locus among them and another 7 restorer lines.The results showed that the amplified bands types were different among the 6 landraces.When compared to the 7 restorer lines,there was the same amplified band type（MW：2.8 kb）,which meant the restorer gene existed in Yunnan landraces had a distant relationship with the restorer lines’using in rice production.So it could be an efficient measure to exploit new restorer genes and resources from Yunnan rice landraces.