| 大花蕙兰茎尖组织培养与植株再生研究 |
| |
| 引用本文： | 徐永清,胡国富,李凤兰,刘荣梅,胡宝忠. 大花蕙兰茎尖组织培养与植株再生研究[J]. 东北农业大学学报, 2009, 40(4) |
| |
| 作者姓名： | 徐永清 胡国富 李凤兰 刘荣梅 胡宝忠 |
| |
| 作者单位： | 1. 东北农业大学成栋学院,哈尔滨,150030
2. 东北农业大学生命科学学院,哈尔滨,150030 |
| |
| 基金项目： | 东北农业大学科学研究基金 |
| |
| 摘 要： | 文章以大花蕙兰为材料,研究其茎尖组织培养再生体系。结果表明,最佳原球茎诱导培养基为VW+0.8mg·L-16-BA+0.2mg·L-1NAA+20g·L-1蔗糖+0.05g·L-1AC,pH5.2。原球茎体分化丛生芽经壮苗生根后可进行移栽,最佳移栽基质为树皮块和水苔藓。
|
| 关 键 词： | 大花蕙兰 组织培养 再生体系 |
Tissue culture and plant regeneration of shoot tip of Cymbidium grandiflorium |
| |
| XU Yongqing,HU Guofu,LI Fenglan,LIU Rongmei,HU Baozhong. Tissue culture and plant regeneration of shoot tip of Cymbidium grandiflorium[J]. Journal of Northeast Agricultural University, 2009, 40(4) |
| |
| Authors: | XU Yongqing HU Guofu LI Fenglan LIU Rongmei HU Baozhong |
| |
| Affiliation: | 1.Chengdong College;Northeast Agricultural University;Harbin 150030;China;2.College of Life Sciences;China |
| |
| Abstract: | The shoot tip of Cymbidium grandiflorium was used as explants to study the tissue culture and regeneration system.The results showed that the optimal medium for protocorm induction was VW + 0.8 mg·L-1 6-BA + 0.2 mg·L-1 NAA + 0.05 g·L-1 AC with 20 g·L-1 sucrose,and pH 5.2.The rooted plantlets derived from protocorm could be transplanted.The optimal transplant mediums were pieces of cortex and lichen. |
| |
| Keywords: | Cymbidium grandiflorium tissue culture regeneration system |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
