| 大花蕙兰茎尖组织培养与植株再生研究 |
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| 引用本文： | 徐永清,胡国富,李凤兰,刘荣梅,胡宝忠.大花蕙兰茎尖组织培养与植株再生研究[J].东北农业大学学报,2009,40(4). |
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| 作者姓名： | 徐永清 胡国富 李凤兰 刘荣梅 胡宝忠 |
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| 作者单位： | 1. 东北农业大学成栋学院,哈尔滨,150030
2. 东北农业大学生命科学学院,哈尔滨,150030 |
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| 基金项目： | 东北农业大学科学研究基金 |
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| 摘 要： | 文章以大花蕙兰为材料,研究其茎尖组织培养再生体系。结果表明,最佳原球茎诱导培养基为VW+0.8mg·L-16-BA+0.2mg·L-1NAA+20g·L-1蔗糖+0.05g·L-1AC,pH5.2。原球茎体分化丛生芽经壮苗生根后可进行移栽,最佳移栽基质为树皮块和水苔藓。
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| 关 键 词： | 大花蕙兰 组织培养 再生体系 |
Tissue culture and plant regeneration of shoot tip of Cymbidium grandiflorium |
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| XU Yongqing,HU Guofu,LI Fenglan,LIU Rongmei,HU Baozhong.Tissue culture and plant regeneration of shoot tip of Cymbidium grandiflorium[J].Journal of Northeast Agricultural University,2009,40(4). |
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| Authors: | XU Yongqing HU Guofu LI Fenglan LIU Rongmei HU Baozhong |
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| Institution: | 1.Chengdong College;Northeast Agricultural University;Harbin 150030;China;2.College of Life Sciences;China |
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| Abstract: | The shoot tip of Cymbidium grandiflorium was used as explants to study the tissue culture and regeneration system.The results showed that the optimal medium for protocorm induction was VW + 0.8 mg·L-1 6-BA + 0.2 mg·L-1 NAA + 0.05 g·L-1 AC with 20 g·L-1 sucrose,and pH 5.2.The rooted plantlets derived from protocorm could be transplanted.The optimal transplant mediums were pieces of cortex and lichen. |
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| Keywords: | Cymbidium grandiflorium tissue culture regeneration system |
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