| 印度圆粒小麦抗条锈病新基因YrSph的遗传分析和SSR标记定位 |
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| 引用本文： | 陈国跃,陈华,魏育明,李伟,刘亚西,刘登才,兰秀锦,郑有良.印度圆粒小麦抗条锈病新基因YrSph的遗传分析和SSR标记定位[J].植物病理学报,2011,41(6):611-617. |
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| 作者姓名： | 陈国跃 陈华 魏育明 李伟 刘亚西 刘登才 兰秀锦 郑有良 |
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| 作者单位： | 四川农业大学小麦研究所， 雅安 625014; 农业部西南作物种质资源利用重点开放实验室， 雅安 625014; 四川农业大学农学院， 雅安 625014 |
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| 基金项目： | “973”计划前期研究专项(2010CB134402) |
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| 摘 要： | 抗条锈病小麦新种质D31是以印度圆粒小麦（Triticum sphaerococcum Perc.）AS384与普通小麦品系94-3854杂交、回交选育而成的新品系。用中国小麦条锈病菌（Puccinia striiformis f.sp. stritici）流行生理小种条中32号对D31和Taichung29的杂交后代进行苗期及成株期抗条锈性遗传分析。结果表明，D31对生理小种条中32号表现为全生育期近免疫抗性反应，其抗性由1对隐性基因控制，暂命名为YrSph。利用BSA法对构建的F2遗传作图群体进行SSR标记分析。通过对400对微卫星引物的筛选和群体分析表明，抗性基因与 Xwmc149、Xwmc246、Xgwm372和Xwmc198 具有连锁关系，其中与 Xwmc246 标记连锁较近，遗传距离为8.8 cM。基因和标记之间的顺序为 Xwmc149-YrSph-Xwmc246-Xgwm372-Xwmc198 。根据作图结果，将D31所含的抗条锈病基因YrSph定位于2A短臂上。基于该基因的作图位置与系谱分析，认为该基因可能是1个新的抗条锈病基因。
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| 关 键 词： | 条锈病抗性基因 染色体定位 微卫星标记 印度圆粒小麦 |
| 收稿时间： | 2011-01-09; |
Genetic analysis and mapping of a stripe rust resistance gene YrSph derived from Triticum sphaerococcum Perc. |
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| CHEN Guo-yue
,
CHEN Hua
,
WEI Yu-ming
,
LI Wei
,
LIU Ya-xi
,
LIU Deng-cai
,
LAN Xiu-jin
,
ZHENG You-liang.Genetic analysis and mapping of a stripe rust resistance gene YrSph derived from Triticum sphaerococcum Perc.[J].Acta Phytopathologica Sinica,2011,41(6):611-617. |
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| Authors: | CHEN Guo-yue CHEN Hua WEI Yu-ming LI Wei LIU Ya-xi LIU Deng-cai LAN Xiu-jin ZHENG You-liang |
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| Institution: | Triticeae Research Institute, Sichuan Agricultural University, Ya’an 625014, China; Key Laboratory of Crop Germplasm Resources Utilization in Southwest China, Ministry of Agriculture, Sichuan Agricultural University, Ya’an, 625014, China; College of Agronomy, Sichuan Agricultural University, Ya’an 625014, China |
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| Abstract: | Stripe rust,caused by Puccinia striiformis f.sp.tritici(PST),is one of the most devastating diseases in common wheat(Triticum aestivum L.).A new common wheat line D31,developed from a cross of AS384(T.sphaerococcum Perc.) and 94-3854,is resistant to the currently prevailing physiological races of PST in China.With the objective of identifying and tagging the new gene for resistance to stripe rust in wheat line D31,F1 and F2 populations from the cross Taichung 29/D31 were inoculated with Chinese PST race CYR... |
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| Keywords: | stripe rust resistant gene chromosomal location microsatellite marker Triticum sphaerococcum Perc |
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