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云南水稻上检测到南方水稻黑条矮缩病毒
引用本文:丁铭,尹跃艳,方琦,韦加贵,顾中量,李红祥,卢训,赵健,张仲凯.云南水稻上检测到南方水稻黑条矮缩病毒[J].植物病理学报,2011,41(6):640-644.
作者姓名:丁铭  尹跃艳  方琦  韦加贵  顾中量  李红祥  卢训  赵健  张仲凯
作者单位:云南省农业科学院生物技术与种质资源研究所, 昆明 650223; 云南省农业科学院高山经济植物研究所, 丽江 674100; 文山州富宁县植保站, 富宁 663400; 德宏州植保站, 潞西 678400; 西双版纳州植保站, 景洪 666100
基金项目:农业部公益性行业科研专项(nyhyzx07-051)
摘    要:南方水稻黑条矮缩病(Southern rice black streaked dwarf disease)是我国南方稻区一种新的水稻病毒病害.自2001年在广东省阳西县首次发现以来,该病危害范围逐年扩大,2009年我国湖南、江西、广东、广西、海南、浙江、福建、湖北和安徽9个水稻主产省(区)及越南北部19个省发病面积约33.33万hm2,基本绝收面积0.67万hm21,2],对水稻生产威胁巨大.

关 键 词:水稻生产  水稻黑条矮缩病毒  分离物  白背飞虱  外壳蛋白基因  禾本科作物  首次发现  云南省  检测方法  西双版纳州  
收稿时间:2010-12-17;

Detection of Southern rice black streaked dwarf virus(SRBSDV) in rice in Yunnan Province
DING Ming , YIN Yue-yan , FANG Qi , WEI Jia-gui , GU Zhong-liang , LIHong-xiang , LU Xun , ZHAO Jian , ZHANG Zhong-kai.Detection of Southern rice black streaked dwarf virus(SRBSDV) in rice in Yunnan Province[J].Acta Phytopathologica Sinica,2011,41(6):640-644.
Authors:DING Ming  YIN Yue-yan  FANG Qi  WEI Jia-gui  GU Zhong-liang  LIHong-xiang  LU Xun  ZHAO Jian  ZHANG Zhong-kai
Institution:Biotechnology and Genetic Gerplasm Resources Institute, YAAS, Kunming 650223, China; The Institute of Alpine Economic Plant, YAAS, Lijiang 674100, China; Plant Protection Station of Funing County , Funing 663400, China; Plant Protection Station of Dehong State; Luoxi 678400, China; Plant Protection Station of Xishuangbanna State, Jinghong 666100, China
Abstract:Rice samples showing symptoms similar to the disease caused by Southern rice black streaked dwarf virus(SRBSDV,Genus Fijivirus,Family Reoviridae) collected from three municipalities of southern Yunnan were detected by electron microscopy and RT-PCR.Electron microscopy observation of crude infected leaf extracts after negative staining revealed spherical particles of 70-75 nm,and about 411 bp fragment of RT-PCR shared 97.6%-100% nucleotide identities with reported capsid protein gene of SRBSDV isolates.The p...
Keywords:Yunnan Province  rice  Southern rice black streaked dwarf virus  RT-PCR  
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