| 三叶草绿变病植原体的分子鉴定 |
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| 引用本文： | 李正男,刘萍,吴云锋. 三叶草绿变病植原体的分子鉴定[J]. 植物病理学报, 2011, 41(6): 645-648 |
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| 作者姓名： | 李正男 刘萍 吴云锋 |
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| 作者单位： | 旱区作物逆境生物学国家重点实验室 植保资源与病虫害治理教育部重点实验室 西北农林科技大学植物保护学院， 杨凌 712100 |
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| 基金项目： | 国家自然科学基金(30970133); 高等学校博士学科点基金(20100204110004); 高等学校学科创新引智计划(B07049) |
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| 摘 要： | 三叶草(Trifolium pratense Linn.)为车轴草属,蝶形花科多年生草本植物,原产亚洲南部和欧洲东南部,是一种世界性分布与栽培的优良牧草.因其花叶兼优、草姿美、绿期长而具有较高的观赏价值,近几年作为草坪用草被广泛种植.在自然条件下,三叶草很容易受到不同种植原体的侵染,国外已报道的侵染三叶草的植原体有:三叶草绿变植原体( Clover phyllody phytoplasma,CPh)和三叶草增殖植原体(Clover proliferation phytoplasma,CP)等,这些植原体分别属于16SrI组和16SrⅥ组[1,2].
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| 关 键 词： | 植原体 枣疯病 绿变 优良牧草 三叶草 |
| 收稿时间： | 2010-12-02; |
Molecular detection and identification of a phytoplasma associated with Clover phyllody |
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| LI Zheng-nan , LIU Ping , WU Yun-feng. Molecular detection and identification of a phytoplasma associated with Clover phyllody[J]. Acta Phytopathologica Sinica, 2011, 41(6): 645-648 |
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| Authors: | LI Zheng-nan LIU Ping WU Yun-feng |
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| Affiliation: | State Key Laboratory of Crop Stress Biology for Arid Areas and Key Laboratory of Plant Protection Resources and Pest Management, Ministry of Education, College of Plant Protection, Northwest A&F University, Yangling 712100, China |
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| Abstract: | Clover phyllody,suspected to be infected by phytoplasma,was observed in Yangling,Shaanxi Province.Using phytoplasma universal 16S rRNA primer pairs R16mF2/R16mR1 and R16F2n/R16R2,the 1.2 kb product of expected size was amplified from clover exhibiting symptoms of phyllody.GenBank BLASTn analysis revealed that the phytoplasma shared highest similarity with members of 16SrV-B subgroup.Phylogenetic tree analysis indicated that the clover phytoplasma clustered together with the phytoplasmas of 16SrV-B subgroup.... |
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| Keywords: | phytoplasma Trifolium pratense Linn. nested PCR phylogenetic tree |
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