Tomato chlorotic dwarf viroid in the ornamental plant Vinca minor and its transmission through tomato seed |
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Authors: | Rudra P. Singh Andrea D. Dilworth |
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Affiliation: | (1) Potato Research Centre, Agriculture and Agri-Food Canada, 850 Lincoln Road, P.O. Box 20280, Fredericton, NB E3B 4Z7, USA |
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Abstract: | Two novel aspects of Tomato chlorotic dwarf viroid (TCDVd) are reported, namely that TCDVd was detected in symptomless plants of Vinca minor, a trailing ground cover surviving at subzero temperatures (−12°C); and that TCDVd was seed-borne in tomato and detected in high percentages in tomato seeds and seedlings. Soaking seeds in a low concentration of sodium hypochlorite did not eliminate the viroid. The sequence analysis showed that the TCDVd isolate consists of 360 nucleotides and has sequence identity between 96% to 99% with isolates of TCDVd from other hosts. |
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Keywords: | Detection Hot-climate pathogen Ornamental plants Survival in cool climates Viroids |
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