| 美洲商陆抗真菌蛋白转化玉米的研究和抗病性检测 |
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| 引用本文： | 刘爽, 杨爱国, 赵琦, 赵玉锦, 张世煌.美洲商陆抗真菌蛋白转化玉米的研究和抗病性检测[J].玉米科学,2007,15(2):031-035. |
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| 作者姓名： | 刘爽 杨爱国 赵琦 赵玉锦 张世煌 |
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| 作者单位： | 1. 首都师范大学生命科学学院,北京,100037
2. 中国农业科学院作物所,北京,100081 |
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| 摘 要： | 研究美洲商陆抗病毒蛋白(PaAFP)基因转入玉米中对玉米纹枯病的抗性。从美洲商陆叶片中获得美洲商陆抗真菌蛋白前体蛋白基因cDNA序列,构建植物表达载体pCAMBIA1300-PaAFP,通过三亲杂交法将其导入根癌农杆菌LBA4404受体菌,转化玉米获得了大量再生转基因植株。PCR、PCR-southern杂交、RT-PCR以及Tris-Tricine-SDS-PAGE检测结果表明,目的基因已经整合到玉米基因组中,并且已经得到转译。
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| 关 键 词： | 玉米 PaAFP前体蛋白基因 抗真菌 立枯丝核菌 |
| 文章编号： | 1005-0906（2007）02-0031-05 |
| 收稿时间： | 2006-04-21 |
| 修稿时间： | 2006-04-212007-01-27 |
Maize Transformation of PaAFP Gene and Disease Resistance Assay |
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| LIU Shuang,YANG Ai-guo,ZHAO Qi,ZHAO Yu-jin,ZHANG Shi-huang.Maize Transformation of PaAFP Gene and Disease Resistance Assay[J].Journal of Maize Sciences,2007,15(2):031-035. |
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| Authors: | LIU Shuang YANG Ai-guo ZHAO Qi ZHAO Yu-jin ZHANG Shi-huang |
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| Institution: | 1. Institute of Life Sciences, Capita] Normal University, Beijing 100037; 2. Institute of Corps, Chinese Academy of Agricultural Sciences, Beijing 100081, China |
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| Abstract: | In the paper, plant expression vector pCAMBIA1300-PaAFP was constructed by cloning the PaAFP gene from pokeweed to pCAMBIA-1300. Then, the engineered strain LBA4404 was constructed by conducting the pCAMBIA-1300-PaAFP to A. tumefaciens LBA4404 and was used to transform maize. The PCR, PCR-Southern blotting, RT-PCR, SDS-PAGE analysis indicated that the PaAFP gene had been integrated into the genome of maize and produced the protein. |
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| Keywords: | Maize PaAFP Antifungal Rhizoctonia solani |
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