| 葡萄的两种检疫性病毒的多重RT-PCR检测 |
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| 引用本文： | 薛杨,安德荣,李明福.葡萄的两种检疫性病毒的多重RT-PCR检测[J].植物病理学报,2005,35(Z1):14-17. |
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| 作者姓名： | 薛杨 安德荣 李明福 |
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| 作者单位： | 1 西北农林科技大学植保学院, 陕西杨凌 712100;2 中国检验检疫科学研究院动植物检疫研究所, 北京 100029 |
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| 摘 要： | 本研究利用多重RT-PCR技术,对葡萄的检疫性病毒中番茄环斑病毒(ToRSV)和烟草环斑病毒(TRSV)进行检测,并研究了反应体系中各个参数对多重RT-PCR的影响。结果表明:可以在同一反应体系中实现对ToRSV和TRSV的RT-PCR检测,但模板浓度、引物浓度、循环数等3个因素对检测结果的准确性有较大的影响,3个参数的增加都会导致非特异性扩增的增多,而dNTP以及Taq酶的浓度对实验结果并无较大影响。
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| 关 键 词： | 多重RT-PCR 番茄环斑病毒(ToRSV) 烟草环斑病毒(TRSV) |
Detection the two quarantine viruses infecting grapevine by multiple RT-PCR |
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| XUE Yang,AN De-rong,LI Ming-fu.Detection the two quarantine viruses infecting grapevine by multiple RT-PCR[J].Acta Phytopathologica Sinica,2005,35(Z1):14-17. |
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| Authors: | XUE Yang AN De-rong LI Ming-fu |
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| Institution: | 1 College of Plants Protection, Northwest Sci-Tech University of Agriculture and Forestry, Yangling, Shaanxi 712100, China;2 Institute of Animal and Plant Quarantine, CAIQ, Beijing 100029, China |
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| Abstract: | Multiple RT-PCR was applied to detect the import quarantine viruses on grapevine: Tomato ring-spot virus (ToRSV), Tobacco ringspot virus (TRSV). The factors affecting RT-PCR reaction of this system were studied,too. The results indicated ToRSV and TRSV could be detected by this method successfully, and three ingredients; template concentration, primer concentration and cycles had more influences on the results in the system than that of dNTP concentration and Taq DNA polymerase concentration. |
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| Keywords: | multiplex RT-PCR Tomato ringspot virus(ToRSV) Tobacco ringspot virus(TRSV) |
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