消化道不同部位灌注大豆寡糖对绵羊几个免疫指标影响

为研究不同剂量大豆寡糖(SBOS)对绵羊体液免疫和细胞免疫功能的影响。试验选用9只安装有永久性瘤胃瘘管、十二指肠瘘管和回肠瘘管的内蒙古半细毛羊羯羊,随机分为3组。试验1组灌注占试验日粮风干物质0.6%的SBOS;试验2组灌注占试验日粮风干物质1.2%的SBOS;对照组不灌注SBOS。试验分3期,分别在瘤胃、十二指肠和回肠3个部位灌注SBOS(每期进行1个部位的灌注)。结果表明:(1)通过瘤胃和十二指肠灌注,SBOS提高了绵羊外周血CD4细胞数量、CD4/CD8值、血清IgG和IgA含量,其中以1.2%组效果最为明显;(2)回肠灌注SBOS,增加了外周血CD8细胞数量,降低了CD4/CD8值;0.6%组增加了CD4细胞数量,提高了IgG、IgA含量;1.2%组降低了CD4细胞数量,在第14天和21天达到了显著水平(P<0.05),并且在14 d和21 d降低了IgG、IgA含量。因此,SBOS可以在一定程度上提高绵羊的免疫功能。

Effects of Soybeanoligosaccharides Infused in Different Parts of the Gastrointestinal Tract on Several Immunity Indexes in Sheep

The objective of this study was to determine the effects of SBOS infused in different parts of the gastrointestinal tract on cell-mediated and humoral immunity function in sheep.Nine Inner Mongolian semi-fine wool wethers fitted with permanent ruminal and proximal duodenum and ileum cannulas were randomly divided into three groups with three sheep each.The treatments were:(1) control group without SBOS;(2) Group 1 infused with SBOS at level of 0.6% of basic diet in rumen daily;(3) Group 2 infused with 1.2% SBOS.This experiment was carried out in three stages,in which SBOS was infused in the rumen,duodenum and ileum,respectively.The results showed that:(1) the amounts of CD4 T-lymphocytes,the ratio of CD4/CD8 in blood,as well as the concentration of serum IgG and IgA were increased when SBOS was infused in the rumen and duodenum with the best effect at 1.2% level(P<0.05);(2) SBOS increased the amount of CD8 T-lymphocytes and decreased the ratio of CD4/CD8 when infused in the ileum.Infusion with 0.6% SBOS in the ileum increased the amount of CD4 and the concentration of serum IgG and IgA.Infusion with 1.2 % SBOS in the ileum significantly reduced the amount of CD4 T-lymphocytes(P<0.05) and lowered the concentration of serum IgG and IgA but without significant difference at 14d and 21d as compared with control group.SBOS could improve cell-mediated and humoral immunity function in sheep when infused in different parts of the gastrointestinal tract.