| 高丛越橘离体叶片再生植株研究初报 |
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| 引用本文： | 马怀宇,李亚东,刘庆忠,赵红军,孙清荣. 高丛越橘离体叶片再生植株研究初报[J]. 东北农业大学学报, 2004, 35(2): 212-215 |
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| 作者姓名： | 马怀宇 李亚东 刘庆忠 赵红军 孙清荣 |
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| 作者单位： | 吉林农业大学园艺学院,长春,130118;山东省果树研究所,泰安,271000 |
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| 基金项目： | 吉林省长春市科技局资助项目，国家外专局资助项目 |
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| 摘 要： | 用越橘组培苗的叶片作为外植体 ,进行不定芽再生植株的研究。以改良的 WPM为其基本培养基 ,添加玉米素5 mg· L- 1 ,不定芽再生率高达 90 %。所用品种中除乔治亚姆外均有相当高的再生率。培养基中的玉米素浓度低于 5mg· L- 1时 ,不定芽的再生率也随之下降 ;基本培养基中加入 TDZ和 BA,再生率只为 0 %～ 30 %。不定芽的增殖、生根、温室锻炼、大田移栽均已获得成功
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| 关 键 词： | 赵橘 离体叶片 再生 不定芽 |
| 文章编号： | 1005-9369(2004)02-0212-04 |
| 修稿时间： | 2003-05-21 |
Regeneration of highbush blurberry plantlets from leaf section |
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| MA Huai-yu ,LI Ya-dong ,LIU Qing-zhong ,ZHAO Hong-jun ,SUN Qing-rong. Regeneration of highbush blurberry plantlets from leaf section[J]. Journal of Northeast Agricultural University, 2004, 35(2): 212-215 |
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| Authors: | MA Huai-yu LI Ya-dong LIU Qing-zhong ZHAO Hong-jun SUN Qing-rong |
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| Affiliation: | MA Huai-yu 1,LI Ya-dong 1*,LIU Qing-zhong 2,ZHAO Hong-jun 2,SUN Qing-rong 2 |
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| Abstract: | Shoot regeneration from in vitro leaf segments of highbush blueberry (Vaccinium corymbosum L.),including four northern highbush blueberry (Sierra,Sunrise,Duke,Elliot)and one southern highbush blueberry (Geogiagem).Modified WPM was used as basic medium supplemented with the cytokinin zeatin.High regeneration frequency was obtained at 5 mg·L -1 zeatin,accounted for 90%.Conversely,medium containing TDZ at 1,2 or 3 mg·L -1 only got very low regeneration percent(0%～30%).And medium with BA at 1,3 or 5 mg·L -1 promoted no adventitious shoots from leaf segments.The adventitious shoots were multiplied and rooted.The plantlets were transplanted into greenhouse and established in the field. |
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| Keywords: | in vitro leaves regeneration adventitious bud |
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