| 铁皮石斛类原球茎增殖和分化的研究 |
| |
| 引用本文： | 林丛发,钟爱清,林云斌,陈芦根.铁皮石斛类原球茎增殖和分化的研究[J].江西农业学报,2007,19(1):84-86,91. |
| |
| 作者姓名： | 林丛发 钟爱清 林云斌 陈芦根 |
| |
| 作者单位： | 福建省宁德市农业科学研究所,福建,宁德,355017 |
| |
| 摘 要： | 通过单因素浓度试验和正交试验研究了5种金属离子、不同激素组合及不同天然附加物对铁皮石斛类原球茎增殖、分化的影响。试验结果表明,5种金属离子对类原球茎增殖影响的主次顺序为Mg2+(Fe2+(Ca2+(Mn2+(Zn2+,类原球茎增殖的最佳培养基为1/2MS+NAA 0.5mg/L+10%马铃薯汁+3%蔗糖(含Mg2+1.0 mmol/L、Ca2+2.0 mmol/L、Fe2+0.1mmol/L、Mn2+0.1mmol/L和Zn2+0.03 mmol/L)。培养基1/2MS+NAA 0.5mg/L+20%马铃薯汁+蔗糖20g/L+琼脂0.8g/L最适于铁皮石斛类原球茎的分化。
|
| 关 键 词： | 铁皮石斛 类原球茎 金属离子 增殖 分化 |
| 文章编号： | 1001-8581（2007）01-0084-03 |
| 收稿时间： | 2006-09-05 |
| 修稿时间： | 2006-09-05 |
Study on Proliferation and Redifferentiation of Protocorm-like Bodies of Dendrobium candidum |
| |
| LIN Cong-fa,ZHONG Ai-qing,LIN Yun-bin,CHEN Lu-gen.Study on Proliferation and Redifferentiation of Protocorm-like Bodies of Dendrobium candidum[J].Acta Agriculturae Jiangxi,2007,19(1):84-86,91. |
| |
| Authors: | LIN Cong-fa ZHONG Ai-qing LIN Yun-bin CHEN Lu-gen |
| |
| Institution: | Institute of Agricultural Sciences of Ningde City, Ningde 355017, China |
| |
| Abstract: | This research is concerned with redifferentiation optimization of five metal ions,different hormone and natural juice for protocorm-like body(PLBs) proliferation in liquid culture of Dendrobium candidum by one factor at a time and orthogonal test.Results showed that,the effects of metal ions on PLBs proliferation were significant and the order was Mg~(2+)﹥Fe~(2+)﹥Ca~(2+)﹥Mn~(2+)﹥Zn~(2+).The optimal medium for PLBs proliferation was determined as 1/2MS+NAA0.5mg/L+10%PE+3%sugar(containing Mg~(2+)1.0 mmol/L,Ca~(2+)2.0 mmol/L,Fe~(2+)0.1 mmol/L,Mn~(2+)0.1mmol/L and Zn~(2+)0.03 mmol/L).The optimal medium for PLBs redifferentiation was determined as 1/2MS+NAA0.5mg/L+20% PE+sugar 20g/L+agar 0.8g/L. |
| |
| Keywords: | Dendrobium candidum Protocorm-like bodies(PLBs) Metal ion Proliferation Redifferentiation |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
