| 玉米基因枪遗传转化体系的研究 |
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| 引用本文： | 肖莉杰,苍晶. 玉米基因枪遗传转化体系的研究[J]. 黑龙江八一农垦大学学报, 2006, 18(3): 13-16 |
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| 作者姓名： | 肖莉杰 苍晶 |
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| 作者单位： | 1. 黑龙江八一农垦大学生命科学技术学院,大庆,163319
2. 东北农业大学生命科学院 |
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| 基金项目： | 国家植物转基因研究与产业化专项课题 |
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| 摘 要： | 以玉米自交系81162的胚性愈伤组织为受体材料,通过基因枪轰击法将nptII基因和afp基因转入玉米细胞,获得了抗性愈伤组织和再生植株。确定了筛选剂Geneticin的筛选浓度和方法,愈伤组织生长阶段和分化阶段Geneticin的有效浓度分别为60mg/L和30mg/L。对2个轰击参数氦气压力与轰击距离进行了组合试验,结果表明1100psi的氦气压力和8cm的轰击距离为最佳组合。PCR检测证明,目的基因已整合到玉米基因组中。
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| 关 键 词： | 玉米 遗传转化 基因枪 |
| 文章编号： | 1002-2090（2006）03-0013-04 |
| 收稿时间： | 2006-03-14 |
| 修稿时间： | 2006-03-14 |
Studies on Genetic Transformation System of Maize by Particle Bombardment |
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| XIAO Li-jie,CANG Jing. Studies on Genetic Transformation System of Maize by Particle Bombardment[J]. journal of heilongjiang bayi agricultural university, 2006, 18(3): 13-16 |
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| Authors: | XIAO Li-jie CANG Jing |
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| Abstract: | By particle bombardment technique, npt II and afp gene were transferred into embryonic callus of inbred line 81162, resistant callus and plants were obtained. The concentration and method of filtration reagent Geneticin were determined, namely 60 mg/L and 30 mg/L in differentiation stage for resistant callus were feasible. Optimization of parameters for gene transformation was analyzed. The result showed that the group of 1 100 psi helium pressure and 8 cm of bombardment distance were the best group of transformation. It was indicated by PCR assay that the aim gene had been integrated into maize genome. |
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| Keywords: | maize transformation particle bombardment |
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