Functional scFv antibody sequences against the organophosphorus pesticide chlorpyrifos |
| |
Authors: | Alcocer M J Doyen C Lee H A Morgan M R |
| |
Institution: | Institute of Food Research, Norwich Research Park, Colney, Norwich NR4 7UA, United Kingdom. |
| |
Abstract: | Two functional single-chain Fv (scFv) antibodies that recognize specifically the widely used organophosphorus pesticide chlorpyrifos-ethyl were derived from two murine hybridoma cell lines. It is shown that the functional scFvs could be isolated without any rounds of selection, with a success rate dependent on the efficiency of amplification of the functional light chain gene family by the specific primers. Besides four new functional immunoglobulin variable gene sequences, the isolation of a new pseudogene is reported. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|