益生菌PSB0201对原代培养罗非鱼肠道上皮细胞的影响

通过分离和原代培养尼罗罗非鱼肠道上皮细胞，并以其为细胞模型，研究目前已广泛用作益生菌的光合细菌PSB0201对其形态、增殖、存活率、通透性、胞浆游离Ca2＋和磷脂酶A2活性的影响。结果表明，与对照组相比，添加一定浓度的光合细菌PSB0201对肠道上皮细胞的形态和各生化指标均有一定影响，但是差异均不显著（P > 0.01）。表明在一定时间内PSB0201对尼罗罗非鱼肠道上皮细胞没有毒害作用，为其作为益生菌安全性的重新评价以及在包括水产养殖在内的农业中的应用提供了新的思路和一定的理论依据。

Effect ofProbiotic PSB0201 on Primary Culture Epithelial Cells of Tilapia (Oreochromis nilotica ) Intestine

Intestinal epithelial cells of tilapias, Oreochromis nilotica, were isolated and cultivated by primary culture of cells as the cells model to research the effect of Photosynthetic Bacteria (PSB0201) treated widely with the probiotic on the epithelial morphologic characters, viability, livability, permeability, cytosolic free calcium and membranous phospholipase A2 (PLA2) activity of tilapias. However, there was no significance （P > 0.01）comparing with the control although there was little effect of definited concentration PSB0201 on the intestine epithelial morphology and biochemical indexes. The result showed no harm on the epithelial cells of tilapia intestine during definite time and provided new idea and partial academic elements to reappraise the safety of PSB0201 treated as probiotic and apply in agriculture including aquaculturet.