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粘红酵母与金属离子结合使用对柑橘采后青霉病的抑制效果
引用本文:孙萍,郑晓冬,张红印,吴忠民,唐平,蒋伟. 粘红酵母与金属离子结合使用对柑橘采后青霉病的抑制效果[J]. 果树学报, 2003, 20(3): 169-172
作者姓名:孙萍  郑晓冬  张红印  吴忠民  唐平  蒋伟
作者单位:浙江大学食品科学与营养系,浙江,杭州,310029
基金项目:国家自然科学基金项目(30170659)。
摘    要:研究了粘红酵母(Rhodotorulaglutinis)不同处理方法对柑橘果实采后病害(指状青霉)的抑制效果。结果表明:粘红酵母细胞悬浮液的抑菌效果最好,当浓度为1×109CFU/mL时,几乎完全抑制病害的发生,而滤液则会使病害加重。当粘红酵母分别与0.1mol/L浓度的Ca2+、0.1mol/L的Mg2+结合使用时,可提高抑菌效果。如与Fe2+使用时,高浓度的Fe2+会加重病害的发生,低浓度对抑菌效果不明显,当仅以浓度为2.5μmol/L的添加量抑菌效果较好。

关 键 词:红酵母  金属离子  柑橘  采后病害  生物防治
文章编号:1009-9980-(2003)-03-169-04
修稿时间:2002-10-09

Effects of Rhodotorula glutinis in Combination With Metal Cation on BioControl of Postharvest Disease in Citrus
Abstract:Effects of different treatments of Rhodotorula glutinis on biocontrol efficiency of postharvest disease (blue mold)were studied on citrus.The washed cells of R.glutinis at1×10 9 CFU(colony-forming unit)/mL provided the best and even the complete biocontrol capacity of postharvest rot on citrus.Cell-free culture filtrate of R.glutinis was not effective in pre-venting the decay and even aggravated the disease.The biocontrol capacity of R.glutinis was improved when inoculated in combination with0.1mol/L calcium or0.1mol/L magnesium in an aqueous suspension.The disease was aggravated when the medium was supplemented with high concentration of iron(FeSO 4 ),but it had no effect in low concentration of iron,the bio-control effect was better when2.5μmol/L iron concentration added in medium.At the same time ,some action mechanism was dis cussed.
Keywords:Rhodotorula glutinis  Metal cation  Citrus  Postharvest disease  Biocontrol
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