| 应用氯化锂法小量提取质粒DNA |
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| 引用本文： | 潘建菁,张君诚,卢艺萍,张杭颖,庄伟建.应用氯化锂法小量提取质粒DNA[J].福建农业学报,2007,22(4):469-470. |
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| 作者姓名： | 潘建菁 张君诚 卢艺萍 张杭颖 庄伟建 |
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| 作者单位： | 1. 福建农林大学作物科学学院,福建,福州,350002
2. 三明学院,福建,三明,365000 |
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| 基金项目： | 国家烟草专卖局资助项目(110200601011) |
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| 摘 要： | 用改良的氯化锂方法小量提取质粒DNA。结果表明,该方法所提取的质粒DNA的质量与常规碱裂解法获得的质粒DNA基本一致,可以满足分子生物学基础实验的要求;同时该方法简化了试验步骤,质粒DNA提取时间缩短了24 min。
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| 关 键 词： | 质粒DNA 氯化锂 |
| 文章编号： | 1008-0384（2007）04-0469-02 |
| 收稿时间： | 2007-02-27 |
| 修稿时间： | 2007-12-12 |
A new method-LiCl for extracting bacterium plasmid DNA |
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| PAN Jian-jing,ZHANG Jun-cheng,LU Yi-ping,ZHANG Hang-ying,ZHUANG Wei-jian.A new method-LiCl for extracting bacterium plasmid DNA[J].Fujian Journal of Agricultural Sciences,2007,22(4):469-470. |
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| Authors: | PAN Jian-jing ZHANG Jun-cheng LU Yi-ping ZHANG Hang-ying ZHUANG Wei-jian |
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| Institution: | 1. Collegeof Crop Science, FujianAgriculture and Forestry University, Fuzhou, Fujian 350002, China; 2. Sanming University, Sanming, Fujian 365000, China |
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| Abstract: | A new method to extract small amount of plasmid DNA with LiCl was developed.The results showed that the quality of plasmid DNA extracted by using this method was basically identical to that of the conventional alkalinelysis.The method satisfied the need for basic molecular biology experiments,and the procedure was simplified shortening the extracting time by 24 min. |
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| Keywords: | Plasmid DNA LiCl |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
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