| 微量荧光法测定血清中维生素A含量 |
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| 引用本文： | 李宏全,华修国,王新红.微量荧光法测定血清中维生素A含量[J].黑龙江畜牧兽医,1994(7):5-6. |
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| 作者姓名： | 李宏全 华修国 王新红 |
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| 作者单位： | 山西农业大学动物医学系，上海农学院动物科学系，山西省晋中地区卫生学校 |
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| 摘 要： | 采用荧光法测定了水牛、奶牛、绵羊、杂种犬、产蛋鸡血清中维生素Ａ的含量，测定值分别为２８．３±２．７、９７．６±８．２、２４．８±４．９、４４．４±３．８、１２１．７±１．４ｕｇ／ｄｌ；用同一血清样品做１０次重复性分析，精确度Ｓ分别为０．０８、０．０６、０．１０、０．１２、０．０８，变异系数≤０．３５％、平均回收率≥９８．８％．试验表明荧光法测定血清中维生素Ａ的最佳激发光波长为３４５ｎｍ、发射光波长为４８０ｎｍ．该方法灵敏度高，试样用量少，操作简便．部分测定值填补了动物血液生化参数的空白．
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| 关 键 词： | 荧光法 血清 维生素A |
A Fluorimeteric Method For Measuring Serum VitamineA oF Animal |
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| Li Hongquan, Hua Xiuguo.A Fluorimeteric Method For Measuring Serum VitamineA oF Animal[J].Heilongjiang Animal Science And veterinary Medicine,1994(7):5-6. |
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| Authors: | Li Hongquan Hua Xiuguo |
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| Institution: | the animal medicine department of Shanxi agricultural university |
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| Abstract: | The serum vitamine A contents of water buffalo,milk cowsheepmongrel dog,laying eggs hen were determined with fluorimetric method in the research and the values were 28.3+2.7, 97.6+8.2, 24.8+4.9, 44.4 + 3.8,121.7 + 1.4ug/dl respectively.the standard errors of 10 times repeated analysis for the same serum samples were 0.08,0.06,0.10,0.12,0.08 respectively,and the coefficient of variation was less than 0.35%.The rate of recovery was more than 98.8%.In this methoed,the excitatiom wavelength 345nm and emission maximum 480nm were selected.The results showed that the method has high sensitivity and simple procedures,some values obtained in the experiment filled in the gaps of animal biood biochemical parameters. |
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| Keywords: | Fluormctric Method Serum vitamine A |
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