| 不同型魏氏梭菌菌体蛋白和膜蛋白SDS-PAGE电泳图谱分析 |
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| 引用本文： | 杨明凡,田永军,崔保安,王学斌,苗舜尧.不同型魏氏梭菌菌体蛋白和膜蛋白SDS-PAGE电泳图谱分析[J].中国兽医杂志,2000,26(11):12-14. |
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| 作者姓名： | 杨明凡 田永军 崔保安 王学斌 苗舜尧 |
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| 作者单位： | 1. 河南农业大学牧医工程学院，河南 郑州 450002
2. 河南省畜牧局，河南 郑州 450003 |
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| 摘 要： | 运用ＳＤＳ－ＰＡＧＥ技术分别对Ａ、Ｂ、Ｃ、Ｄ四个型魏氏梭菌的菌体蛋白和细菌膜蛋白进行分析。结果在菌体蛋白图谱中Ａ型缺少５４．９ＫＤ蛋白条带，Ｃ型缺少１０７．８ＫＤ和３６．１ＫＤ蛋白条带，Ｄ型缺少１０７．８ＫＤ和６６．６ＫＤ蛋白条带。在膜蛋白图谱中仅Ｂ型有９４．７ＫＤ蛋白条带但缺少５１ＫＤ蛋白条带，Ｃ型缺少７４．２ＫＤ蛋白条带，Ｄ型缺少３１ＫＤ蛋白条带，但比其它３个型多１个２４．３ＫＤ蛋白条带，且３
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| 关 键 词： | 魏氏梭菌 菌体蛋白 膜蛋白 SDPS－PAGE 电泳图谱 |
| 文章编号： | 0529-6005(2000)11-0012-03 |
| 修稿时间： | 1999年7月29日 |
SDS-PAGE analysis of cell proteins and membrane proteins of Clostridium perfringens |
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| YANG Ming fan ,TIAN Yong jun ,CUI Bao an ,WANG Xue bin ,MIAO Shun yao.SDS-PAGE analysis of cell proteins and membrane proteins of Clostridium perfringens[J].Chinese Journal of Veterinary Medicine,2000,26(11):12-14. |
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| Authors: | YANG Ming fan TIAN Yong jun CUI Bao an WANG Xue bin MIAO Shun yao |
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| Institution: | YANG Ming fan 1,TIAN Yong jun 2,CUI Bao an 1,WANG Xue bin 1,MIAO Shun yao 1 |
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| Abstract: | The cell proteins of different types of Cl. perfringens produce reproducible electrophoretic patterns. In the electrophoretic patterns of cell proteins Cl. perfringens type A didn't have the 54.9 KD band; type C didn't have the 107.8 KD and 36.2 KD bands and type D didn't have the 107.8 KD and 66.6 KD bands. So the electrophoretic patterns of cell proteins in polyacrylamide gel were used for the rapid identification and classification of Cl. perfringens. And the electrophoretic patterns of membrane proteins of the different Cl. perfringens were specific. In the electrophoretic patterns of membrane proteins only type B had the 94.7 KD band but didn't have the 51 KD band; type C didn't have the 74.2 KD band; only type D had the 24.3 KD band but didn't have the 31 KD band and the 38.1 KD band was faint. This method may also be used for the identification of Cl. perfringens . |
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| Keywords: | Clostridium perfringens cell proteins membrane proteins Sodium Dodecyl SulphatePolyacrylamide Gel Electrophoresis |
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