| 转大豆铁结合蛋白基因水稻的初步研究 |
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| 引用本文： | 王彩芬,付永彩,安永平,韩国敏,张文银,马静. 转大豆铁结合蛋白基因水稻的初步研究[J]. 西北农业学报, 2008, 17(6): 84-87 |
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| 作者姓名： | 王彩芬 付永彩 安永平 韩国敏 张文银 马静 |
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| 作者单位： | 1. 宁夏农林科学院,农作物研究所,宁夏永宁,750105
2. 中国农业大学,植物遗传育种系,农业生物技术国家重点实验室,农业部农作物基因组学与遗传改良重点开放实验室,北京,100094 |
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| 基金项目： | 宁夏回族自治区自然科学基金 |
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| 摘 要： | 采用冻融法将含有大豆铁结合基因的质粒pGTV-35S-fer转入农杆菌菌株LBA4404中,以宁夏4个水稻主栽品种为受体材料,通过农杆菌介导法转化水稻愈伤组织,经2轮次除草剂筛选获得了再生植株。经过对转化植株的PCR扩增检测,证明外源大豆铁结合基因已经转入宁夏水稻植株中。大豆铁结合蛋白基因在水稻种子中特异表达,转基因植株种子中的铁含量是未转化植株的1.7~2.2倍。
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| 关 键 词： | 大豆铁结合基因 农杆菌转化 水稻 |
| 收稿时间： | 2008-03-03 |
| 修稿时间： | 2008-04-18 |
Preliminary Study on the Soybean Ferritin Transgenic Rice |
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| WANG Cai-fen,FU Yong-cai,AN Yong-ping,HAN Guo-min,ZHANG Wen-yin and MA Jing. Preliminary Study on the Soybean Ferritin Transgenic Rice[J]. Acta Agriculturae Boreali-occidentalis Sinica, 2008, 17(6): 84-87 |
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| Authors: | WANG Cai-fen FU Yong-cai AN Yong-ping HAN Guo-min ZHANG Wen-yin MA Jing |
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| Affiliation: | Crop Institute of Ningxia Agriculture and Forestry Academy, Yongning Ningxia 750105, China;Department of Plant Genetic and Breeding, China Agricultural University, State Key Laboratory for Agrobiotechnology;Key Laboratory of Crop Improvement and Genome of Ministry of Agriculture, Beijing 100094, China;Crop Institute of Ningxia Agriculture and Forestry Academy, Yongning Ningxia 750105, China;Crop Institute of Ningxia Agriculture and Forestry Academy, Yongning Ningxia 750105, China;Crop Institute of Ningxia Agriculture and Forestry Academy, Yongning Ningxia 750105, China;Crop Institute of Ningxia Agriculture and Forestry Academy, Yongning Ningxia 750105, China |
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| Abstract: | The plasmid with pGTV-35S-fer was introduced into Agrobacterium strain LBA4404 by freeze-thaw method.The primary callus derived frommature embryos of four Ningxia rice verities was used for transformation.Under the selection on basta-containing medium,transgenic rice plants were obtained.PCR analysis of total DNA from primary transformants confirmed that soybean ferritin gene integrated into the rice plants.The result showed that the ferritin gene could specifically express in the endosperm of transgenic rice,and the iron content in the endosperm of transgenic rice was 1.7~2.2 times higher than that of the untransformed wild type plant. |
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| Keywords: | Soybean ferritin gene Transformation by Agrobacterium-mediated pathway Rice |
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