| 葡萄溃疡病菌原生质体的制备与再生条件的优化 |
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| 引用本文： | 张玮,燕继晔,郭霞,李兴红. 葡萄溃疡病菌原生质体的制备与再生条件的优化[J]. 植物保护, 2012, 38(5): 27-30 |
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| 作者姓名： | 张玮 燕继晔 郭霞 李兴红 |
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| 作者单位： | 北京市农林科学院植物保护环境保护研究所,北京,100097 |
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| 摘 要： | 对葡萄溃疡病菌菌丝摇培时间、酶解时间、渗透压稳定剂种类及再生培养基类型进行了探索和优化,建立了葡萄溃疡病菌稳定、高效的原生质体制备体系。以SR培养基作为再生培养基时,原生质体再生率达到最高,为32.2%。该研究为建立葡萄溃疡病菌高效、稳定的遗传转化体系,开展其致病机理的研究奠定了基础。`
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| 关 键 词： | 葡萄溃疡病菌 原生质体 制备与再生条件 |
Development of a method for protoplast preparation and regeneration of Botryosphaeria rhodina |
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| Zhang Wei,Yan Jiye,Guo Xi,Li Xinghong. Development of a method for protoplast preparation and regeneration of Botryosphaeria rhodina[J]. Plant Protection, 2012, 38(5): 27-30 |
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| Authors: | Zhang Wei Yan Jiye Guo Xi Li Xinghong |
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| Affiliation: | (Institute of Plant and Environment Protection,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100097,China) |
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| Abstract: | The culture time of mycelium, the enzymolysis time, the type of osmotic stabilizer and the regeneration medium were optimized in this study. An efficient method for protoplast preparation and regeneration of Botryosphaeria rhodina was obtained. While SR medium was chosen as the regeneration medium, the regeneration rate of protoplast reached the highest, up to the maximum of 32.2%. This study provided powerful support for genetic transformation and transformant pathogenicity analysis in Botryosphaeria spp. |
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| Keywords: | Botryosphaeria spp. protoplast preparation and regeneration |
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