重要植物寄生线虫内切葡聚糖酶基因研究进展

 在线虫与植物互作的过程中，降解寄主细胞壁是植物线虫成功建立寄生关系的关键环节。β-1,4-内切葡聚糖酶(β-1,4-endoglucanase,ENG)是由线虫食道腺细胞产生并由口针分泌、对细胞壁降解起关键作用的酶类之一。本文对近年来植物寄生线虫eng基因的克隆、组织定位、表达分析、基因、编码蛋白的结构功能以及ENG来源、进化及在线虫与植物互作中的潜在作用等进行了概述。

Progress on endoglucanase gene of key plant parasitic nematodes

During interaction between the plant and its parasitic nematodes,host plant cell wall degradation is the key step for plant nematode parasitism.The β-1,4-endoglucanases(ENG),secreted from the esophageal gland cells of plant parasitic nematodes(PPN),can hydrolyze cellulose that is one of the main components of plant cell walls.This paper gives a brief review on the major research advances of nematode ENG which include its gene cloning,tissue localization,expression analysis,gene and protein structure and function analysis,gene evolution based on ENG sequences and the potential role during plant and PPN interactions.