大豆茎溃疡病菌子囊壳诱导及孢子萌发方法探索

大豆茎溃疡病是为害大豆的重要病害,世界上十大大豆生产国中已有7个国家发现大豆茎溃疡病菌的分布,对当地农业造成严重威胁1]。该病是我国进境植物检疫性有害生物2],目前没有发生为害的报道。大豆茎溃疡病菌以菌丝和子囊壳在大豆植株及其残体上越冬,子囊壳在干燥35 d

Method of the perithecium producing and ascospore germination of Diaporthe phaseolorum

Diaporthe phaseolorum is a potential dangerous fungus to soybean.Determination method for obtaining a big amount of ascospores and a reliable spore germination rate is the base of quarantine treatment and control.On the basis of acquiring quantities of perithecium of D.phaseolorum,a new method of ascospore germination with perithecium as the observation unit was explored.In the test,the traditional suspended drops method and water agar surface germination method were used as reference.The results showed that the germination rate of perithecium germination method was lower than that of traditional method in the earlier stage(12 h,24 h,36 h),then became in accordance with the traditional method gradually.So,for pathogenetic fungi which can easily produce quantities of perithecium on the host organization and difficultly obtain ascospore,perithecium germination method has reference value on spore germination rate determination.