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| 一种适合于富含多糖和酚类物质的香蕉果实RNA提取方法 | |
| 引用本文： | 刘海,林德球,徐杰,蒋跃明.一种适合于富含多糖和酚类物质的香蕉果实RNA提取方法[J].果树学报,2006,23(1):136-137. |
| 作者姓名： | 刘海 林德球 徐杰 蒋跃明 |
| 作者单位： | 1. 中国科学院华南植物园,广州,510650;华南师范大学生命科学院,广州,510631;中国科学院研究生院,北京,100039 2. 华南师范大学生命科学院,广州,510631 3. 中国科学院华南植物园,广州,510650 |
| 基金项目： | 国家高技术研究发展计划(863计划) |
| 摘 要： | 酚类化合物和多糖是影响香蕉组织RNA提取的几个主要干扰因素。研究通过在裂解液中加入聚乙烯吡咯烷酮去除多酚类化合物和利用水饱和乙醚去除多糖,改进了香蕉果实RNA的提取方法。该方法具有快速、简单等特点。 |
| 关 键 词： | 香蕉 果实 RNA 提取 多糖 多酚类化合物 |
| 文章编号： | 1009-9980（2006）01-136-02 |
| 收稿时间： | 2004-09-07 |
| 修稿时间： | 2005-11-22 |
A Simple and rapid method for extracting RNA from banana fruit pulp rich in polysaccharides and polyphenolic compounds |
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| LIU Hai,LIN De-qiu,XU Jie,JIANG Yue-ming.A Simple and rapid method for extracting RNA from banana fruit pulp rich in polysaccharides and polyphenolic compounds[J].Journal of Fruit Science,2006,23(1):136-137. | |
| Authors: | LIU Hai LIN De-qiu XU Jie JIANG Yue-ming |
| Institution: | 1 South China Botanic Garden, Chinese Academy of Science, Guangzhou, Guangdong 510650 China; 2 Biotech Research Institute, South China Normal University, Guangzhou , Guangdong 510631 China; 3 Graduate School, Chinese Academy of Science, Beijing 100039 China |
| Abstract: | Extraction of high-quality RNA from banana fruit pulp tissues is particularly difficult due to high levels of polyphenol and polysaccharides. A suitable method for extracting RNA from banana fruit pulp has been improved by added polyvinylpyrrolidone into the extract solution to remove polyphenolic compounds and using water-saturated ether to remove polysaccharides. In this study, a high-quality RNA from banana fruit pulp tissues was obtained by using the improved method. The procedure was simple and rapid. |
| Keywords: | Banana Fruit RNA Extraction Polysaccharides Polyphenolic compounds |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |