| 利用MPCR技术快速检测进口大豆转基因背景 |
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| 引用本文： | 徐景升,余爱丽,姚伟,饶进,张木清,陈如凯. 利用MPCR技术快速检测进口大豆转基因背景[J]. 江西农业大学学报, 2005, 27(2): 262-264,269 |
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| 作者姓名： | 徐景升 余爱丽 姚伟 饶进 张木清 陈如凯 |
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| 作者单位： | 福建农林大学,农业部甘蔗生理生态与遗传改良重点开放实验室,福建,福州,350002 |
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| 基金项目： | 福建省重大国际合作项目(2001I002)，948项目“甘蔗良种和技术引进与推广”(2003-Q06)，863高新技术发展计划课题(2001AA241191) |
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| 摘 要： | 利用改良的CATB方法提取大豆(Glycine max)种子基因组DNA。以大豆内源ScII基因做对照，采用MPCR(Multiplex Polymerase Chain Reaction，MPCR)技术从进口大豆中检测出35S启动子、NOS终止子和EPSPS耐除草剂基因3种转基因成分。
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| 关 键 词： | MPCR 转基因 大豆 检测 |
| 文章编号： | 1000-2286(2005)02-0262-03 |
Rapid Detection of Imported Genetically Modified Soybean (Glycine max) with MPCR |
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| XU Jing-sheng,YU Ai-li,YAO Wei,Rao Jin,ZHANG Mu-qing,CHEN Ru-kai. Rapid Detection of Imported Genetically Modified Soybean (Glycine max) with MPCR[J]. Acta Agriculturae Universitis Jiangxiensis, 2005, 27(2): 262-264,269 |
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| Authors: | XU Jing-sheng YU Ai-li YAO Wei Rao Jin ZHANG Mu-qing CHEN Ru-kai |
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| Abstract: | The DNA was successfully extracted from soybean(Glycine max) seeds by a modified CTAB method. Three transgenic elements, 35S promoter, NOS terminator and EPSPS (5-enolpyruvylshikimate-3- phosp-hate synthase), were detected in the imported soybean seeds by MPCR(Multiplex Polymerase Chain Reaction,MPCR) with the ScII as the control. |
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| Keywords: | MPCR transgenic soybean(Glycine max) detection |
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