| 影响木薯叶片蛋白质双向电泳图谱的因素分析 |
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| 引用本文： | 安飞飞,张振文,郑永清,李庚虎. 影响木薯叶片蛋白质双向电泳图谱的因素分析[J]. 热带农业科学, 2011, 31(5): 33-37 |
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| 作者姓名： | 安飞飞 张振文 郑永清 李庚虎 |
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| 作者单位： | 1. 中国热带农业科学院品种资源研究所,海南儋州,571737
2. 海南大学农学院,海南海口,570228 |
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| 基金项目： | 现代农业产业技术体系(No.CARS-12); 973项目(No.2010CB126606); 中央级公益性科研院所基本科研业务费专项(No.PZS-062) |
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| 摘 要： | 以木薯华南205为试材,采用苯酚抽提法提取叶片蛋白质,并通过双向电泳技术研究影响木薯叶片蛋白电泳分析图谱的主要因素。结果表明：pH 4～7的IPG胶条可提高特定范围蛋白质点的分辨率;上样量200μg可得到较好的双向电泳图谱。本研究不仅为进一步研究木薯叶片蛋白电泳分析提供技术手段,同时也为分析木薯块根、茎和繁殖器官的蛋白...
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| 关 键 词： | 木薯叶片 双向电泳 IPG胶条 |
Influencing Factors of Protein Two-dimensional Electrophoresis in Cassava Leaf |
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| AN Feifei) ZHANG Zhenwen) ZHENG Yongqing) LI Genghu). Influencing Factors of Protein Two-dimensional Electrophoresis in Cassava Leaf[J]. Chinese Journal of Tropical Agriculture, 2011, 31(5): 33-37 |
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| Authors: | AN Feifei) ZHANG Zhenwen) ZHENG Yongqing) LI Genghu) |
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| Affiliation: | AN Feifei1) ZHANG Zhenwen1) ZHENG Yongqing1) LI Genghu2)(1 Tropical Crops Genetic Resources Institute,CATAS,Danzhou,Hainan 571737,2 College of Agronomy,Hainan University,Haikou,Hainan 570228) |
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| Abstract: | South China 205(SC105) was adopted in this experiment to compare different effects of two-dimensional electrophoresis(2-DE) gels.Phenol extraction method was used to extract proteins.The results showed that immobilized pH gradients(IPG) strip with pH 4~7 can improve protein resolution in certain range.Sample loading of 200 mg produced better 2-DE gel image.The results not only provides techniques for getting a good 2-DE profile of cassava leaf,also for tuberous root,stem and other reproduction organ. |
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| Keywords: | cassava leaf 2-dimensional electrophoresis(2-DE) immobilized pH gradients(IPG) strip |
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