| 双链RNA技术与植物病毒研究 |
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| 引用本文： | 张晓婷,吴祖建,林奇英,谢联辉. 双链RNA技术与植物病毒研究[J]. 云南农业大学学报(自然科学版), 2005, 20(4): 455-458 |
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| 作者姓名： | 张晓婷 吴祖建 林奇英 谢联辉 |
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| 作者单位： | 福建农林大学植物病毒研究所 福建福州350002(张晓婷，吴祖建，林奇英)，福建农林大学植物病毒研究所 福建福州350002(谢联辉) |
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| 摘 要： | 90%以上的植物病毒基因组是RNA。含RNA基因组的病毒会产生特异的双链RNA（dsRNA）。从受到病毒侵染的植物组织中提取病毒相关的dsRNA，进行病毒的检测和分类，诊断植物病毒病，探索生物防治植物病毒病的新途径，是植物病毒研究的重要内容。本文就双链RNA技术在植物病毒研究中的应用做一综述。
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| 关 键 词： | 双链RNA 植物病毒 |
| 文章编号： | 1004-390X(2005)04-0455-04 |
| 收稿时间： | 2004-06-15 |
dsRNA Isolation Method and Research of Plant Virology |
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| ZHANG Xiao-ting,WU Zu-jian,LIN Qi-ying,XIE Lian-hui. dsRNA Isolation Method and Research of Plant Virology[J]. Journal of Yunnan Agricultural University, 2005, 20(4): 455-458 |
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| Authors: | ZHANG Xiao-ting WU Zu-jian LIN Qi-ying XIE Lian-hui |
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| Affiliation: | （Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou 350002，China） |
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| Abstract: | dsRNA isolation method, widely used in virology, is a basic technique for dsRNA research. In tissues of plants infected by viruses, dsRNA is specific for each of them.So the extraction of dsRNA can be used to detect whether a virus exists. And we can also try to find ways to control the desease. The dsRNA isolation method is very useful in the research of plant virology. Recent years, this technique has developed greatly and its application has expanded a lot. The development has been discussed, and the advantages or disadvantages have been analysed. Also, its aboard usage in diagnosis and taxonomy of viruses, gene engineering and biocontrol has been reviewed here. |
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| Keywords: | dsRNA plant virus |
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