几个籼型杂交水稻恢复系的抽穗期基因型诊断

以基因型明确的抽穗期主基因近等基因系EG0~EG7(检测E1、E2和E3位点)、ER~LR(检测Se-1位点)、T65系列(检测Ef-1位点)为测验系,在江西南昌(28°68′N)自然长日照(14~16 h/d)和人工遮光短日照(10h/d)诱导处理条件下,对籼型杂交水稻恢复系“早25”、“974”和“752”的抽穗期基因型进行了诊断分析。结果表明,“早25”的抽穗期基因型为e1e1E2E2E3E3Se-1nSe-1nef-1ef-1,“974”的抽穗期基因型为e1e1E2E2E3E3Se-1eSe-1eEf-1aEf-1a,同时存在感光基因表达的抑制因子i-Se-1,“752”的抽穗期基因型为E1E1E2E2E3E3Se-1eSe-1eEf-1bEf-1b。

Genotype Diagnosis of Heading Date in Three Restoring Lines of Indica Hybrid Rice (Oryza sativa)

The genotypes diagnoses of heading date in three restoring lines of indica hybrid breeding rice "Zao 25","974" and "752" with special reference to photoperiod sensitivity loci E_1,E_2,E_3,Se-1 and basic vegetative growth period loci Ef-1 were performed.The segregation patterns of days from sowing to heading of parents,F_1 and F_2 generations from the crosses of the restoring lines with nearly isogenic lines,EG0~EG7,ER~LR,T65,T65m,T65 E~b and T65 E~bm,whose genotypes of heading date were closed and clearly known,were subjected to heading date genetic identification under natural long day length and short day length(10h/d) treatment by sunshade in Nanchang,Jiangxi province.Results showed that the genotype of heading date in "Zao 25" was e_1e_1E_2E_2E_3E_3Se-1~nSe-1~nef-1ef-1,in "974" was e_1e_1E_2E_2E_3E_3Se-1~eSe-1~eEf-1~aEf-1~a,meanwhile existed a recessive photoperiod sensitivity inhibitor gene i-Se-1,in "752" was E_1E_1E_2E_2E_3E_3Se-1~eSe-1~eEf-1~bEf-1~b.