大豆蛋白质提取工艺中碱溶pH值的简单效应分析

研究了提取大豆蛋白质工艺中pH值对蛋白质提取率及得率的简单效应,结果表明:①在料水比1∶10、酸沉pH值4.3、离心速度4 000 r/min、碱溶pH值9.5、碱溶时间为45 min的条件下,蛋白质平均提取率最高为66.49%。在pH值7.5~9.0之间,碱溶pH值对蛋白质提取率的简单效应为2.88%;在pH值9.0~10.0之间,简单效应下降直至为负值。方差分析表明,各处理间蛋白质提取率差异极显著;新复极差测验表明,pH值7.5~9.0之间时,各处理的蛋白质提取率差异极显著,pH值9.0~10.0之间时,差异不显著。②同样条件下,蛋白质平均得率最高为37.23%。在pH值7.5~9.0之间,碱溶pH值对蛋白质得率的简单效应为2.33%;在pH值9.0~10.0之间,简单效应下降直至为负值。方差分析表明,各处理间蛋白质得率达显著水平;新复极差测验表明,pH值7.5~8.5之间时,各处理间的蛋白质得率差异显著,pH值8.5~10.0之间时,差异不显著。③在不考虑其他因素时,为了取得碱溶pH值的最大简单效应,宜选择9.0作为碱溶pH值参数。

Analysis on Simple Effect of Alkali Soluble pH Value in the Extraction Process of Soybean Protein

The simple effect of alkali soluble pH value on the extraction rate of soybean protein(ERSP)and yield rate of soybean protein(YRSP) was studied.The results showed that:①When the ratio of material to water was 1∶10,acid precipitation pH was 4.3,centrifugal speed was 4 000 r/min,alkali-soluble pH value was 9.5 and the alkali-soluble was 45 min,the highest mean of ERSP was 66.49%.Among pH 7.5~9.0,the simple effect of alkali soluble pH value on the ERSP was 2.88%.However,among pH 9.0~10.0,the simple effect reduced to a minus value.Analysis of variance showed discrepancy of ERSP among different treatments reached very notable level;The shortest significant range showed,when pH was 7.5~9.0,discrepancy of ERSP reached very notable level,when pH was 9.0~10.0,it was no difference.②When in the same conditions,the highest mean of YRSP was 37.20%.Among pH 7.5~9.0,the simple effect of alkali soluble pH value on the YRSP was 2.33%.The simple effect also reduced among pH 9.0~10.0.Analysis of variance showed discrepancy of ERSP among different treatments reached notabale level;The shortest significant range showed when pH was 7.5~8.5,discrepancy of YRSP was significant difference,when pH was 8.5~10.0,it was no difference.③In order to obtain the biggest simple effect of alkali soluble pH value,pH 9.0 was suggested in the extraction process of soybean protein.