Electrophoretic pattern of proteins isolated from immatured and matured stages of 10 candidate plus trees of versatile oleaginous legume, <Emphasis Type="Italic">Pongamia pinnata</Emphasis> (L.) Pierre

Pongamia pinnata an oil yielding leguminous tree is an economically important plant due to its multifunctional characteristics. The objective of the present study was to investigate the electrophoretic spectra sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) of total soluble seed proteins under reduced conditions (5 mM β-mercaptoethanol) in immatured 135 days after flowering (DAF)] and matured stages (350 DAF) for 10 tagged candidate plus trees (CPTs) of P. pinnata from North Guwahati. SDS-PAGE analysis revealed presence of ~15–20 unique polypeptide fragments of molecular size ranging from 14 to 150 kDa in both stages of seed development but no variations was observed at a particular stage among 10 CPTs. However, polypeptide banding pattern of immatured and fully ripened matured seeds showed differences in expression pattern of three main polypeptide bands (MW 50, 18 and 14 kDa) for all the 10 CPTs studied. Globulins may be considered to be the main seed storage proteins in Pongamia that includes legumins and vicilins.